Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DISSOCIATION OF TETRAMERIC IONS OF NONCOVALENT STREPTAVIDIN COMPLEXESFORMED BY ELECTROSPRAY-IONIZATION
Autore:
SCHWARTZ BL; BRUCE JE; ANDERSON GA; HOFSTADLER SA; ROCKWOOD AL; SMITH RD; CHILKOTI A; STAYTON PS;
Indirizzi:
BATTELLE MEM INST,PACIFIC NW LABS,DEPT CHEM SCI,CHEM METHODS & SEPARAT GRP,POB 999 RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,DEPT CHEM SCI,CHEM METHODS & SEPARAT GRP RICHLAND WA 99352 UNIV WASHINGTON,CTR BIOENGN SEATTLE WA 98195
Titolo Testata:
Journal of the American Society for Mass Spectrometry
fascicolo: 6, volume: 6, anno: 1995,
pagine: 459 - 465
SICI:
1044-0305(1995)6:6<459:DOTION>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLISION-ACTIVATED DISSOCIATION; RESONANCE MASS-SPECTROMETER; PROTEIN; BIOMOLECULES; SUBSTRATE; MYOGLOBIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Schwartz et al., "DISSOCIATION OF TETRAMERIC IONS OF NONCOVALENT STREPTAVIDIN COMPLEXESFORMED BY ELECTROSPRAY-IONIZATION", Journal of the American Society for Mass Spectrometry, 6(6), 1995, pp. 459-465

Abstract

The noncovalent tetrameric association of the protein streptavidin formed by electrospray ionization (ESI) mass spectrometry has been observed intact and dissociated in the gas phase. An extended mass-to-charge ratio range quadrupole mass spectrometer was employed to examine theeffects of harsher conditions in the ESI atmosphere-vacuum interface region on the streptavidin tetramer. Thermally induced dissociation caused the mass spectra to exhibit a series of complementary monomer andtrimer ions that correspond to decomposition of the tetrameric species. Similar results were obtained with tandem mass spectrometric experiments on a Fourier transform ion cyclotron resonance mass spectrometerby application of sustained off-resonance irradiation (SORI) on a selected tetrameric charge state. The technique of single-frequency quadrupole excitation was used to accomplish selected-ion accumulation of the 14 + charge state of the tetramer during ion injection. Subsequent low energy SORI combined with broadband quadrupole cooling produced the 7 + monomer and 7 + trimer species, as well as the 6 + monomer and 8+ trimer complementary ions. The observed asymmetric breakup of the tetramer is qualitatively explained by using physical models.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:04:04