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Titolo:
METABOLIC CONTROL ANALYSIS OF THE PENICILLIN BIOSYNTHETIC-PATHWAY IN A HIGH-YIELDING STRAIN OF PENICILLIUM-CHRYSOGENUM
Autore:
NIELSEN J; JORGENSEN HS;
Indirizzi:
TECH UNIV DENMARK,CTR PROC BIOTECHNOL,DEPT BIOTECHNOL DK-2800 LYNGBY DENMARK
Titolo Testata:
Biotechnology progress
fascicolo: 3, volume: 11, anno: 1995,
pagine: 299 - 305
SICI:
8756-7938(1995)11:3<299:MCAOTP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOPENICILLIN-N SYNTHETASE; STREPTOMYCES-CLAVULIGERUS; ACV SYNTHETASE; DELTA-(L-ALPHA-AMINOADIPYL)-L-CYSTEINYL-D-VALINE SYNTHETASE; CEPHALOSPORIN BIOSYNTHESIS; ACID ACYLTRANSFERASE; ASPERGILLUS-NIDULANS; PURIFICATION; ENZYME; ACREMONIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J. Nielsen e H.S. Jorgensen, "METABOLIC CONTROL ANALYSIS OF THE PENICILLIN BIOSYNTHETIC-PATHWAY IN A HIGH-YIELDING STRAIN OF PENICILLIUM-CHRYSOGENUM", Biotechnology progress, 11(3), 1995, pp. 299-305

Abstract

Metabolic control analysis is used to identify the rate-limiting stepin the penicillin biosynthetic pathway in Penicillium chrysogenum. The analysis is carried out using a kinetic model for the first two steps in the pathway, i.e., the ACV synthetase (ACVS) and the isopenicillin N synthetase (IPNS). The kinetic model is based on Michaelis-Menten type kinetics, with noncompetitive inhibition of the ACVS by ACV and competitive inhibition of the IPNS by glutathione. From measurements ofthe enzyme activities and intracellular metabolites during a fed-batch cultivation, the kinetic model is used to predict the flux through the pathway. The model prediction corresponds well with the measured rate of penicillin biosynthesis. From measurement of the activity of theacyl-CoA:isopenicillin acyltransferase, which catalyzes the third andlast reaction in the pathway, it is concluded that the rate-limiting step is either at the ACVS or at the IPNS. From the kinetic model, theelasticity coefficients and the flux control coefficients are calculated throughout the fed-batch cultivations, and it is found that there is a shift in the flux control from the ACVS to the IPNS during the cultivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 13:22:05