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Titolo:
DEVELOPMENT OF A POLYMER-ENZYME IMMUNOASSAY METHOD AND ITS APPLICATION
Autore:
LIU F; LIU FH; ZHUO RX; PENG Y; DENG YZ; ZENG YE;
Indirizzi:
ROOM 302,BLDG 3 BEIJING 100080 PEOPLES R CHINA WUHAN UNIV,DEPT CHEM WUHAN 430072 PEOPLES R CHINA
Titolo Testata:
Biotechnology and applied biochemistry
, volume: 21, anno: 1995,
parte:, 3
pagine: 257 - 264
SICI:
0885-4513(1995)21:<257:DOAPIM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
F. Liu et al., "DEVELOPMENT OF A POLYMER-ENZYME IMMUNOASSAY METHOD AND ITS APPLICATION", Biotechnology and applied biochemistry, 21, 1995, pp. 257-264

Abstract

Both poly(N-isopropylacrylamide) and poly(N-isopropylacrylamide)-antibody (PINP-Ab)-labelled enzyme adhered quickly and tightly to cellulose acetate/nitrate membrane either below (less efficiently) or above (more efficiently) the lower critical solution temperature, and the retention of PINP-Ab on the membrane increased over 30-fold when compared with the unconjugated Ab. These characteristics were used to develop anovel polymer-enzyme-linked immunoassay method: homogeneous antigen-antibody immune-complexation reaction and a heterogeneous separation process. By using a simple horseradish-peroxidase-labelled antibody as aprobe, we applied this method to the detection of human serum hepatitis B surface antigen (HBsAg). This immunoassay system can detect as little as 1 ng/ml of HBsAg. The advantages of this method are: (a) fast homogeneous immune complexation; (b) a rapid heterogeneous separation process; (c) high sensitivity; and (d) low non-specific background.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:41:55