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Titolo:
ANALYSIS OF PLASMA AND URINARY TEA POLYPHENOLS IN HUMAN-SUBJECTS
Autore:
LEE MJ; WANG ZY; LI H; CHEN LS; SUN Y; GOBBO S; BALENTINE DA; YANG CS;
Indirizzi:
RUTGERS STATE UNIV,COLL PHARM,CANC RES LAB,FRELINGHUYSEN RD PISCATAWAY NJ 08855 RUTGERS STATE UNIV,COLL PHARM,CANC RES LAB PISCATAWAY NJ 08855 THOMAS J LIPTON INC ENGLEWOOD CLIFFS NJ 07632
Titolo Testata:
Cancer epidemiology, biomarkers & prevention
fascicolo: 4, volume: 4, anno: 1995,
pagine: 393 - 399
SICI:
1055-9965(1995)4:4<393:AOPAUT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN TEA; A/J MICE; TUMOR INITIATION; INDUCED LUNG; TUMORIGENESIS; SKIN; CARCINOGENESIS; PROTECTION; INHIBITION; WATER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Lee et al., "ANALYSIS OF PLASMA AND URINARY TEA POLYPHENOLS IN HUMAN-SUBJECTS", Cancer epidemiology, biomarkers & prevention, 4(4), 1995, pp. 393-399

Abstract

Tea has been shown to inhibit chemically induced tumorigenesis in many animal models, but the effects of tea consumption on human carcinogenesis are not conclusive, In order to develop biomarkers for tea consumption, we developed methods for the analysis of tea polyphenols in human plasma and urine samples using HPLC with the coulochem electrode array detection system, (-)-Epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (EGG), and (-)-epicatechin (EC) are the major polyphenols in green tea, Most of the tea polyphenols were in their conjugated forms in the plasma and urine, The samples were incubated with a mixture of beta-glucuronidase and sulfatase to generate the free form of tea polyphenols, After extraction into ethyl acetate and separation by reversed-phase chromatography, EGCG, EGG, and EC were identified on the basis of their retention times and electrochemical characteristics. Due to the high selectivity of the detection mode, interference was minimized, Good quantitative relationshipswere established for a large concentration range of tea polyphenols, The limits of detection for EGCG, EGG, EGG, and EC were from 0.5 to 1.5 ng/ml of plasma or urine sample, After ingestion of 1.2 g of decaffeinated green tea in warm water, the plasma samples collected at 1 h from 4 human volunteers contained 46-268 ng/ml of EGCG, 82-206 ng/ml of EGG, and 48-80 ng/ml of EC, ECG was not detected in plasma samples, The maximum urinary excretion of EGC and EC occurred at 3-6 h, Most of the EGC and EC were excreted in the first 9 h, and the cumulative urinary excretions in the first 24 h were 2.8-3.2 mg of EGC and 1.6-2.3 mg of EC. EGCG and ECG were not detected in the urine samples, Plasma andurinary levels of EGG, EC, and EGCG may be useful markers for quantifying human ingestion of tea and exposure to tea polyphenols, This information is important for studying the effects of tea consumption on human cancers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 07:55:51