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Titolo:
TRANSMISSIBLE RETROVIRUS IN EPSTEIN-BARR VIRUS-PRODUCER B95-8 CELLS
Autore:
SUN R; GROGAN E; SHEDD D; BYKOVSKY AF; KUSHNARYOV VM; GROSSBERG SE; MILLER G;
Indirizzi:
YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM,333 CEDAR ST NEW HAVENCT 06520 YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM NEW HAVEN CT 06520 YALE UNIV,SCH MED,DEPT PEDIAT NEW HAVEN CT 06520 YALE UNIV,SCH MED,DEPT EPIDEMIOL & PUBL HLTH NEW HAVEN CT 06520 MED COLL WISCONSIN,DEPT MICROBIOL MILWAUKEE WI 53226
Titolo Testata:
Virology
fascicolo: 2, volume: 209, anno: 1995,
pagine: 374 - 383
SICI:
0042-6822(1995)209:2<374:TRIEVB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
BURKITTS-LYMPHOMA; SQUIRREL-MONKEY; MONOCLONAL-ANTIBODIES; NUCLEOTIDE-SEQUENCE; MARMOSET LEUKOCYTES; EXPRESSION; GENOME; LINE; IDENTIFICATION; MONONUCLEOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R. Sun et al., "TRANSMISSIBLE RETROVIRUS IN EPSTEIN-BARR VIRUS-PRODUCER B95-8 CELLS", Virology, 209(2), 1995, pp. 374-383

Abstract

Epstein-Barr virus (EBV) released from the B95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of EBV. Here we identify and characterize a retrovirus carried by many cultures of B95-8 cells. The experiments were stimulated by the isolation of a cDNA clone from B95-8 cells in which sequences from the EBV large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, SMRV-H. However, among 413 amino acids predicted from the nucleotide sequence of the gag region of the B95-8 SMRV isolate there were 48 amino acid changes that distinguished this virus from SMRV-H originally isolated from a human lymphoid cell line by Oda et al. (1988, Virology 167, 468-476). Nucleic acid and antibody probes were developed for the B95-8 isolate of SMRV. Using such probes, we found that SMRV-B95-8 was readily transmissible, independent of EBV, as an infectious virus to human B and T cell lines. SMRV-B95-8 was highly fusogenic in the presence or absence of EBV. The ultrastructural appearance of the B95-8 retrovirus was characteristic of a type D retrovirus. Cells dually infected with EBV and SMRV-B95-8 did notdemonstrate increased levels of lyric EB viral replication. SMRV-B95-8 did not by itself cause lymphocyte immortalization or enhance immortalization by EBV. Thus SMRV-B95-8 does not contribute to the major biologic properties of the B95-8 strain of EBV. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:30:33