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Titolo:
PEDIOCOCCUS-ACIDILACTICI IDHD GENE - CLONING, NUCLEOTIDE-SEQUENCE, AND TRANSCRIPTIONAL ANALYSIS
Autore:
GARMYN D; FERAIN T; BERNARD N; HOLS P; DELPLACE B; DELCOUR J;
Indirizzi:
UNIV CATHOLIQUE LOUVAIN,GENET MOLEC LAB,UNITE GENET,PL CROIX SUD 5 B-1348 LOUVAIN BELGIUM UNIV CATHOLIQUE LOUVAIN,GENET MOLEC LAB,UNITE GENET B-1348 LOUVAIN BELGIUM
Titolo Testata:
Journal of bacteriology
fascicolo: 12, volume: 177, anno: 1995,
pagine: 3427 - 3437
SICI:
0021-9193(1995)177:12<3427:PIG-CN>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-LACTATE DEHYDROGENASE; RATE-DEPENDENT REGULATION; ELONGATION-FACTOR-TS; ESCHERICHIA-COLI; STREPTOCOCCUS-LACTIS; MESSENGER-RNA; BACILLUS-STEAROTHERMOPHILUS; LACTOBACILLUS-PLANTARUM; FORMATE DEHYDROGENASE; ACID DEHYDROGENASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
D. Garmyn et al., "PEDIOCOCCUS-ACIDILACTICI IDHD GENE - CLONING, NUCLEOTIDE-SEQUENCE, AND TRANSCRIPTIONAL ANALYSIS", Journal of bacteriology, 177(12), 1995, pp. 3427-3437

Abstract

The gene encoding D-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of Pediococcus acidilactici DNA by complementationfor growth under anaerobiosis of an Escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant. The nucleotide sequence of IdhD encodes a protein of 331 amino acids (predicted molecular mass of 37,210 Da) which shows similarity to the family of D-2-hydroxyacid dehydrogenases. The enzyme encoded by the cloned fragment is equally active on pyruvate and hydroxypyruvate, indicating that the enzyme has both D-lactate and D-glycerate dehydrogenase activities. Three other open reading frames were found in the 2.9-kb insert, one of which (rpsB) is highly similar to bacterial genes coding for ribosomal proteinS2. Northern (RNA) blotting analyses indicated the presence of a 2-kbdicistronic transcript of IdhD (a metabolic gene) and rpsB (a putative ribosomal protein gene) together with a l-kb monocistronic rpsB mRNA. These transcripts are abundant in the early phase of exponential growth but steadily fade away to disappear in the stationary phase. Primer extension analysis identified two distinct promoters driving either cotranscription of ldhD and rpsB or transcription of rpsB alone.

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Documento generato il 03/12/20 alle ore 14:34:32