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Titolo:
ACID-PHOSPHATASE-ACTIVITY IN MATING-TYPE-I AND MATING-TYPE-II CELL-LINES OF PARAMECIUM-PRIMAURELIA
Autore:
RAMOINO P; BELTRAME F; FATO M; MARCENARO G;
Indirizzi:
UNIV GENOA,IST ZOOL,VIA BALBI 5 I-16126 GENOA ITALY UNIV GENOA,DIST I-16145 GENOA ITALY
Titolo Testata:
European journal of histochemistry
fascicolo: 2, volume: 39, anno: 1995,
pagine: 157 - 164
SICI:
1121-760X(1995)39:2<157:AIMAMC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAUDATUM;
Keywords:
MATING TYPE DIFFERENCES; CULTURE LIFE; CYTOMETRY; CELL IMAGE ANALYSIS; CILIATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
P. Ramoino et al., "ACID-PHOSPHATASE-ACTIVITY IN MATING-TYPE-I AND MATING-TYPE-II CELL-LINES OF PARAMECIUM-PRIMAURELIA", European journal of histochemistry, 39(2), 1995, pp. 157-164

Abstract

The cellular acid phosphatase content, a marker enzyme for lysosomal activity, in Paramecium primaurelia mating type I and mating type II cells was determined by optical laser scanning microscopy. The naphtholAS-TR phosphatase-hexazotized pararosaniline method was used to visualize acid phosphatase activity by the light microscopy. Cell lines of both mating types were tested during culture life, from the early log phase to the death phase. The amount of acid phosphatase was higher inmating type II than in mating type I until the onset of the stationary phase, and then the values reversed. Indeed, during the log phase ofgrowth, mating type II cells formed a higher number of food vacuoles,so that, by taking up a higher amount of bacteria, they sooner becamedeprived of food. It is suggested that, by lacking nutrients, their synthesis activities and acid phosphatase content were reduced as compared with mating type I cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:30:14