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Titolo:
ISOFORMS OF PLATELET-DERIVED GROWTH-FACTOR AND ITS RECEPTORS IN EPIRETINAL MEMBRANES - IMMUNOLOCALIZATION TO RETINAL PIGMENTED EPITHELIAL-CELLS
Autore:
VINORES SA; HENDERER JD; MAHLOW J; CHIU C; DEREVJANIK NL; LAROCHELLE W; CSAKY C; CAMPOCHIARO PA;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,WILMER OPHTHALMOL INST,825 MAUMENEE BLDG,600 N WOLFE ST BALTIMORE MD 21287 NIH BETHESDA MD 20892
Titolo Testata:
Experimental Eye Research
fascicolo: 6, volume: 60, anno: 1995,
pagine: 607 - 619
SICI:
0014-4835(1995)60:6<607:IOPGAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR GENE-EXPRESSION; SMOOTH-MUSCLE CELLS; PROLIFERATIVE VITREORETINOPATHY; DIABETIC-RETINOPATHY; PDGF-B; MONOCLONAL-ANTIBODIES; LOCALIZATION; FIBROBLASTS; PROTEINS; GLIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Vinores et al., "ISOFORMS OF PLATELET-DERIVED GROWTH-FACTOR AND ITS RECEPTORS IN EPIRETINAL MEMBRANES - IMMUNOLOCALIZATION TO RETINAL PIGMENTED EPITHELIAL-CELLS", Experimental Eye Research, 60(6), 1995, pp. 607-619

Abstract

Epiretinal membranes (ERMs) form on the inner surface of the retina in conjunction with various ocular disease processes, but the factors controlling their development are not understood. The predominant cell types involved are retinal pigmented epithelial (RPE) cells and retinal glia. Cultured RPE cells secrete platelet-derived growth factor (PDGF), which is chemotactic and mitogenic for both RPE cells and retinal glia and, therefore, could be involved in the development of ERMs. In the present study, we performed immunohistochemical staining for PDGF A chain (PDGF-A), PDGF B chain (PDGF-B), and both types of PDGF receptors (PDGF(r alpha) and PDGF(r beta)) on ERMs associated with various disease processes. PDGF-A is detected in most ERMs, regardless of the associated disease process, and it appears to be localized predominantly in RPE cells, recognized by the presence of pigment and the immunohistochemical demonstration of some or all of the following RPE-associated epitopes: class III beta-tubulin, keratin, the 65-kDa microsomal protein recognized by the RPE9 antibody, and cellular retinaldehyde-binding protein. PDGF-B is found only in minor subpopulations of cells in about half of the ERMs evaluated and, with only occasional exceptions,appears to be localized almost entirely in brood-borne cells found inand around vessels in vascularized ERMs. Both PDGF(r alpha) and PDGF(r beta) are demonstrated in most ERMs with neither isotype consistently predominating: they are found predominantly on RPE cells with many cells expressing both receptor types. ERMs with little or no RPE cell component contain little or no PDGF and PDGF receptor, whereas those inwhich the RPE cell represents the major cell type, have widespread PDGF and PDGF receptor positivity. These findings show that RPE cells inERMs produce PDGF-A and PDGF(alpha) and PDGF(beta) receptors and suggest that autocrine and paracrine stimulation with PDGF may be involvedin ERM pathogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:56:10