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Titolo:
PURIFICATION AND CHARACTERIZATION OF THE ALKENE MONOOXYGENASE FROM NOCARDIA-CORALLINA B-276
Autore:
MIURA A; DALTON H;
Indirizzi:
UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 5, volume: 59, anno: 1995,
pagine: 853 - 859
SICI:
0916-8451(1995)59:5<853:PACOTA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYLOCOCCUS-CAPSULATUS BATH; SOLUBLE METHANE MONOOXYGENASE; ENZYMATIC OMEGA-OXIDATION; PSEUDOMONAS-OLEOVORANS; UTILIZING BACTERIA; METHYLOSINUS-TRICHOSPORIUM; MECHANISTIC IMPLICATIONS; HYDROXYLASE COMPONENT; REDUCTASE COMPONENT; EPOXIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
A. Miura e H. Dalton, "PURIFICATION AND CHARACTERIZATION OF THE ALKENE MONOOXYGENASE FROM NOCARDIA-CORALLINA B-276", Bioscience, biotechnology, and biochemistry, 59(5), 1995, pp. 853-859

Abstract

Alkene monooxygenase from the propene utilizer Nocardia corallina B-276 was separated into three components, and all components were purified to homogeneity and their properties were examined, The epoxidase, with a molecular mass of 95kDa, was considered to catalyze the oxidation of the substrate propene to propylene oxide, It consisted of 53- and35-kDa subunits, which contained approximately 2-mol of non-heme ironper mole of protein. The reductase, molecular mass 40 kDa, was found to contain an FAD and an Fe-2 S-2 cluster, A third protein, which we have called the coupling protein, with a mass of 14 kDa, appears to function as a regulator of activity. The purified AMO system required NADH as an electron donor, and catalyzed alkene epoxidation only, Acetylene, a specific inhibitor for methane monooxygenase, did not inhibit the AMO activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:58:49