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Titolo:
EXPRESSION OF ATP NUCLEOTIDE 3'-PYROPHOSP HOKINASE GENE FROM STREPTOMYCES IN ESCHERICHIA-COLI AND ITS EFFECTS ON HOST-CELLS
Autore:
EZAKI S; TOYONO T; MUTA S; MUKAI JI;
Indirizzi:
KYUSHU UNIV,GRAD SCH GENET RESOURCES TECHNOL,MOLEC GENE TECH LAB FUKUOKA 812 JAPAN
Titolo Testata:
Nippon Nogei Kagakukaishi
fascicolo: 6, volume: 69, anno: 1995,
pagine: 695 - 702
SICI:
0002-1407(1995)69:6<695:EOAN3H>2.0.ZU;2-#
Fonte:
ISI
Lingua:
JPN
Soggetto:
DNA; SEQUENCE; PROTEINS; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
S. Ezaki et al., "EXPRESSION OF ATP NUCLEOTIDE 3'-PYROPHOSP HOKINASE GENE FROM STREPTOMYCES IN ESCHERICHIA-COLI AND ITS EFFECTS ON HOST-CELLS", Nippon Nogei Kagakukaishi, 69(6), 1995, pp. 695-702

Abstract

Several actinomycetes produce nucleotide 3'-pyrophosphokinase (EC 2.7.6.4), which transfers the 5'-beta, gamma-pyrophosphoryl group of ATP or dATP to a variety of purine and pyrimidine nucleotides at their 3'-OH site. To elucidate the expression mechanism and to attempt the use of a gene coding for this enzyme in a heterologous host, we expressed the gene from Streptomyces morookaensis in Escherichia coli. A DNA fragment that included this gene was inserted downstream of the lac promoter in a multicopy plasmid, pUC 119. The resultant plasmid, pUP 3, wasintroduced into E. coli JM 109. When the cells were incubated in the presence of isopropyl-thio-beta-D-galactopyranoside (IPTG), the enzymewas found to be secreted into periplasmic space. E. coli cells harboring pUP 312, derived from pUP 3, grew slowly in the presence of IPTG. 3'-Pyrophosphoryl nucleotides were detected in the nucleotide pools ofthe E. coli cells that produced this enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 07:14:03