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Titolo:
SUBSTRATE PROPERTIES OF C'-METHYL UTP DERIVATIVES IN T7 RNA-POLYMERASE REACTIONS - EVIDENCE FOR N-TYPE NTP CONFORMATION
Autore:
TUNITSKAYA VL; RUSAKOVA EE; PADYUKOVA NS; ERMOLINSKY BS; CHERNYI AA; KOCHETKOV SN; LYSOV YP; MIKHAILOV SN;
Indirizzi:
RUSSIAN ACAD SCI,VA ENGELHARDT MOL BIOL INST,VAVILOV STR 32 MOSCOW 117984 RUSSIA RUSSIAN ACAD SCI,VA ENGELHARDT MOL BIOL INST MOSCOW 117984 RUSSIA
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 400, anno: 1997,
pagine: 263 - 266
SICI:
0014-5793(1997)400:3<263:SPOCUD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA;
Keywords:
T7 RNA POLYMERASE; C'-METHYL ANALOG OF UTP; TRANSCRIPTION; RNA CHAIN TERMINATOR; CONFORMATIONAL ENERGY CALCULATION; SUBSTRATE CONFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
V.L. Tunitskaya et al., "SUBSTRATE PROPERTIES OF C'-METHYL UTP DERIVATIVES IN T7 RNA-POLYMERASE REACTIONS - EVIDENCE FOR N-TYPE NTP CONFORMATION", FEBS letters, 400(3), 1997, pp. 263-266

Abstract

The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhibitor of T7 RNAP while 2'Me-UTPwas shown to terminate RNA chain synthesis. Conformational analysis of the analogues and parent nucleotide using the force-field method indicates that the allowed conformation of UTP during its incorporation into the growing RNA chain by T7 RNAP is limited to the chi angle rangeof 192-256 degrees of N-type conformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:44:03