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Titolo:
COMPARATIVE IMMUNOREACTIVITY OF MOUSE AND HAMSTER SPERM PROTEINS RECOGNIZED BY AN ANTI-P26H HAMSTER SPERM PROTEIN
Autore:
BEGIN S; BERUBE B; BOUE F; SULLIVAN R;
Indirizzi:
MAISONNEUVE ROSEMONT MED CTR,5415 BOUL ASSOMPT MONTREAL PQ H1T 2M4 CANADA MAISONNEUVE ROSEMONT MED CTR MONTREAL PQ H1T 2M4 CANADA UNIV MONTREAL,FAC MED,DEPT OBSTET & GYNECOL MONTREAL PQ H3C 3J7 CANADA
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 41, anno: 1995,
pagine: 249 - 256
SICI:
1040-452X(1995)41:2<249:CIOMAH>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EGG ZONA PELLUCIDA; SURFACE GALACTOSYLTRANSFERASE; MAMMALIAN SPERM; FERTILIZATION INVITRO; ACROSOME REACTION; BINDING INVITRO; RECEPTOR; GLYCOPROTEINS; INHIBITION; IDENTIFICATION;
Keywords:
SPERMATOZOA; PROTEIN; ANTIGEN; FERTILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S. Begin et al., "COMPARATIVE IMMUNOREACTIVITY OF MOUSE AND HAMSTER SPERM PROTEINS RECOGNIZED BY AN ANTI-P26H HAMSTER SPERM PROTEIN", Molecular reproduction and development, 41(2), 1995, pp. 249-256

Abstract

The binding of the spermatozoon to the zona pellucida is a species-specific phenomenon. We have previously shown that the binding of hamster sperm to the homologous zona pellucida involves a sperm 26-kDa glycoprotein, the P26h, originating in the epididymis. In order to establish to what extent this sperm protein is involved in the species-specific recognition of the egg's extracellular coat, we have compared the inhibitory properties of anti-P26h antibodies in a sperm-zona pellucida assay using hamster and mouse gametes. Anti-P26h IgGs inhibit, in a dose-dependent manner, gamete interactions in both species, although in a less efficient manner in the mouse than in the hamster. While anti-26kDa Fab fragments are as efficient as the intact IgG to inhibit hamster sperm-zona pellucida binding, they have no effect on mouse gamete interaction. ELISA, Western blot, and immunohistochemical experiments have been performed in order to characterize the mouse antigen(s) recognized by the anti-P26h antiserum. ELISA and Western blots showed that this antiserum recognized two proteins on mouse spermatozoa that are less reactive than the hamster P26h. These antigens are localized in the acrosomal region of epididymal spermatozoa of both species. These results indicate that the hamster P26H involved in zona pellucida interaction has certain unique epitopes, while others are common to the sperm of both species. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:38:18