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Titolo:
EFFECT OF DIETARY RESTRICTION ON PARTIAL HEPATECTOMY-INDUCED LIVER-REGENERATION OF AGED F344 RATS
Autore:
CHOU MW; SHADDOCK JG; KONG J; HART RW; CASCIANO DA;
Indirizzi:
NATL CTR TOXICOL RES JEFFERSON AR 72079
Titolo Testata:
Cancer letters
fascicolo: 2, volume: 91, anno: 1995,
pagine: 191 - 197
SICI:
0304-3835(1995)91:2<191:EODROP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALORIC RESTRICTION; CELL-PROLIFERATION; CARBON-TETRACHLORIDE; CARCINOGENESIS; DNA; METABOLISM; CYTOCHROME-P-450; AFLATOXIN-B1; EXPOSURE; BINDING;
Keywords:
DIETARY RESTRICTION; PARTIAL HEPATECTOMY; DNA ADDUCT; BENZO[A]PYRENE; AFLATOXIN B-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M.W. Chou et al., "EFFECT OF DIETARY RESTRICTION ON PARTIAL HEPATECTOMY-INDUCED LIVER-REGENERATION OF AGED F344 RATS", Cancer letters, 91(2), 1995, pp. 191-197

Abstract

Fourteen weeks-old male F344 rats maintained on a reduced calorie diet (60% of ad libitum (AL) food consumption) for 6 weeks or for 14 months did not affect the hepatic cell proliferation in terms of % S phasepopulation, determined by evaluation of DNA synthesis in hepatocytes isolated from either young (5 months) or aged (18 months) rats. However, hepatic basal cellular DNA synthesis estimated by [H-3]thymidine incorporation was reduced through acute dietary restriction (DR) in young rats, but increased in aged animals after 14 months restriction. Partial hepatectomy (PH) on aged rats stimulated hepatocyte regeneration and restored some aging-associated biochemical functions, such as drugmetabolizing enzyme-dependent xenobiotic metabolic activation which was determined by measuring the formation of carcinogen-DNA adducts. Forty-eight hours after partial hepatectomy, the % of S phase populationand the basal nuclear DNA synthesis of hepatocytes isolated from the partial hepatectomized DR-rats were 4- and 2.8-fold, respectively, greater than those of hepatocytes from AL-animals. DR reduced aflatoxin B-1 (AFB(1)) metabolizing enzyme activity and decreased the AFB(1)-DNA adduct formation in young rats treated with AFB(1). In aged AL-rats, the formation of AFB(1)-DNA adducts diminished to the same level as that of DR-groups and probably was due to the faster decline of drug metabolizing enzymes in aging AL-rats. However, 48 h after PH, the metabolic activation of AFB(1) was restored in AL- and DR-groups which resulted in the increase of AFB(1)-DNA binding by 4.2 and 1.9-fold, respectively. During the liver regeneration of old PH-rats, DR inhibited the AFB(1)-DNA adduct formation after the PH-rats received a single dose ofAFB(1). DR increased benzo[a]pyrene (BaP) metabolic activation in both young and aged rats. Aging also decreased BaP-DNA adduct formation in both DR and AL-rats. The increase of BaP-DNA adduct formation in PH-groups was attributed to the restoration of BaP-metabolizing enzyme activity during liver regeneration. The PH-stimulated BaP-DNA adduct formation in AL- and DR-rats was 3.4- and 2.0-fold greater than control aged rats, Our results indicated that the stimulation of PH-induced liver regeneration by DR in aged animals may be attributed to the retardation of aging by DR and the retention of more active biochemical and enzymological functions in old DR-animals,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:24:38