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Titolo:
EOSINOPHIL PENETRATION THROUGH CULTURED HUMAN AIRWAY EPITHELIAL-CELL LAYER
Autore:
MASUDA T; YAMAYA M; SHIMURA S; HOSHI H; SASAKI H; SHIRATO K;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 1,AOBA KU,1-1 SEIRYO MACHI SENDAI MIYAGI 980 JAPAN TOHOKU UNIV,SCH MED,DEPT INTERNAL & GERIATR MED SENDAI MIYAGI JAPAN
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 6, volume: 12, anno: 1995,
pagine: 633 - 641
SICI:
1044-1549(1995)12:6<633:EPTCHA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR BASIC-PROTEIN; INTERCELLULAR-ADHESION MOLECULE-1; PLATELET-ACTIVATING-FACTOR; VASCULAR ENDOTHELIAL-CELLS; TRANSENDOTHELIAL MIGRATION; TRACHEAL EPITHELIUM; TIGHT JUNCTIONS; INVITRO; CHEMOTAXIS; ADHERENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
T. Masuda et al., "EOSINOPHIL PENETRATION THROUGH CULTURED HUMAN AIRWAY EPITHELIAL-CELL LAYER", American journal of respiratory cell and molecular biology, 12(6), 1995, pp. 633-641

Abstract

We investigated the mechanisms of eosinophil penetration and mannitolpermeability through a multilayer of cultured human tracheal epithelial cells. Wells of tissue culture plates were separated into the upperand the lower chambers by the cultured epithelial cell layer. Cr-51-labeled eosinophils or H-3-mannitol were put into the lower chamber. Tostimulate the epithelial cells, platelet-activating factor (PAF) and/or phorbol myristate acetate (PMA) were added to the upper chamber. After 4 h of incubation, the eosinophil penetration rate was determined as a percentage of the total count added to the lower chamber. PMA significantly increased the eosinophil penetration rate in a dose-dependent manner (4.0% at 10(-5) M), compared with control (0.67%), whereas PAF itself did not. Activation of eosinophils by the addition of PAF tothe lower chamber produced a significant increase in the eosinophil penetration (6.5% at 10(-6) M), which was inhibited by staurosporine. For determining the mannitol permeability, PMA, PAF and/or supernatant from eosinophils were added to both upper and lower chambers and incubated for 30 min. PMA induced a significant increase in the mannitol permeability (175% of controls at 10(-5) M), whereas PAF itself did not alter it. Supernatant from eosinophils activated by PAF (10(-6) M) significantly increased the permeability (451% of controls), which was blocked by staurosporine. Supernatants from AA861 (a 5-lipoxygenase inhibitor)-treated or phenidon (a phospholipase A(2) inhibitor)-treated eosinophils activated by PAF failed to alter the supernatant-induced increases in mannitol permeability. These findings suggest that substances released from activated eosinophils (not arachidonates) induce an increase in eosinophil penetration and/or airway permeability across epithelium, probably through the activation of protein kinase C in epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 19:15:10