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Titolo:
COMPATIBLE SOLUTES IN THE THERMOPHILIC BACTERIA RHODOTHERMUS-MARINUS AND THERMUS-THERMOPHILUS
Autore:
NUNES OC; MANAIA CM; DACOSTA MS; SANTOS H;
Indirizzi:
UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,APARTADO 127 P-2780 OEIRAS PORTUGAL UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL P-2780 OEIRAS PORTUGAL UNIV COIMBRA,DEPT BIOQUIM P-3000 COIMBRA PORTUGAL
Titolo Testata:
Applied and environmental microbiology
fascicolo: 6, volume: 61, anno: 1995,
pagine: 2351 - 2357
SICI:
0099-2240(1995)61:6<2351:CSITTB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE QUANTUM NMR; HALOPHILIC EUBACTERIA; OSMOTIC-STRESS; SALT CONCENTRATIONS; ESCHERICHIA-COLI; HOT-SPRINGS; S-MIGUEL; OSMOREGULATION; OSMOADAPTATION; ARCHAEBACTERIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
O.C. Nunes et al., "COMPATIBLE SOLUTES IN THE THERMOPHILIC BACTERIA RHODOTHERMUS-MARINUS AND THERMUS-THERMOPHILUS", Applied and environmental microbiology, 61(6), 1995, pp. 2351-2357

Abstract

C-13 nuclear magnetic resonance spectroscopy and H-1 nuclear magneticresonance spectroscopy were used to identify and quantify the organicsolutes of several strains of halophilic or halotolerant thermophilicbacteria. Two strains of Rhodothermus marinus and four strains of ''Thermus thermophilus'' grown in complex medium containing NaCl were examined. 2-O-Mannosylglycerate was a major compatible solute in all strains: the Thermus strains accumulated the beta-anomer only, whereas both anomers were found in R. marinus. 2-O-beta-mannosylglycerate and 2-O-alpha-mannosylglycerate were the major compatible solutes in R. marinus. The former was the predominant solute in cells grown in 2.0 and 4.0% NaCl-containing medium, while the latter was the predominant compatible solute at higher salinities. Glutamate, trehalose, and glucose were also present as minor components. The intracellular K+ concentration, as determined by K-39 nuclear magnetic resonance spectroscopy, in R. marinus increased with salinity and was sufficient to balance the negative charges of the mannosylglycerate. In addition to 2-O-beta-mannosylglycerate, trehalose was a major compatible solute of ''T. thermophilus.'' 2-O-beta-Mannosylglycerate was the main solute in medium containing 1.0 or 2.0% NaCl, while trehalose predominated in cells grown inmedium supplemented with 3.0 or 4.0% NaCI. Glycine betaine, in lower concentrations, was also detected in two ''T. thermophilus'' strains. This is the first report of mannosylglycerate as a compatible solute in bacteria.

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Documento generato il 22/09/20 alle ore 09:46:45