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Titolo:
MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN (MTP) REGULATION IN HEPG2 CELLS - INSULIN NEGATIVELY REGULATES MTP GENE-EXPRESSION
Autore:
LIN MCM; GORDON D; WETTERAU JR;
Indirizzi:
BRISTOL MYERS SQUIBB,DEPT METAB DIS,POB 4000 PRINCETON NJ 08543 BRISTOL MYERS SQUIBB,DEPT METAB DIS PRINCETON NJ 08543
Titolo Testata:
Journal of lipid research
fascicolo: 5, volume: 36, anno: 1995,
pagine: 1073 - 1081
SICI:
0022-2275(1995)36:5<1073:MTTP(R>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEIN; APO-B SECRETION; GROWTH FACTOR-I; APOLIPOPROTEIN-B; RAT HEPATOCYTES; DISULFIDE-ISOMERASE; CHOLESTEROL HOMEOSTASIS; DIETARY-CHOLESTEROL; PLASMA-LIPOPROTEINS; MONOCLONAL-ANTIBODY;
Keywords:
PLASMA LIPOPROTEINS; APOLIPOPROTEIN B; VERY LOW DENSITY LIPOPROTEINS; LIPID TRANSFER PROTEIN; PROTEIN DISULFIDE ISOMERASE; INSULIN-LIKE GROWTH FACTOR-I;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
M.C.M. Lin et al., "MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN (MTP) REGULATION IN HEPG2 CELLS - INSULIN NEGATIVELY REGULATES MTP GENE-EXPRESSION", Journal of lipid research, 36(5), 1995, pp. 1073-1081

Abstract

The microsomal triglyceride transfer protein (MTP) is a heterodimericlipid transfer protein that is required for the assembly and secretion of apoB-containing lipoproteins. In this study, four factors that modulate lipid and lipoprotein metabolism were tested for their ability to regulate MTP levels in HepG2 cells. Of the factors tested, only insulin (greater than or equal to 10(-9) M), and high concentrations of glucose (>30 mM) were found to decrease MTP large subunit mRNA levels. Oleate and glucagon had no effect on MTP mRNA levels. The insulin effect was dose- and time-dependent and was mediated through the insulin receptor. In addition, insulin also decreased protein disulfide isomerase (the small subunit of MTP) mRNA levels, although to a lesser extent. Due to the slow turnover rate of MTP (t(1/2) = 4.4 days), short-terminsulin treatment (24 h) did not change MTP activity levels, indicating that the regulation of MTP mRNA levels by insulin is unrelated to insulin's acute inhibition of apoB secretion in HepG2 cells. In summary, MTP mRNA levels are acutely regulated by insulin in HepG2 cells; however, sustained changes in MTP mRNA levels would be required to affectMTP protein levels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:00:04