Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMMUNOLOGICAL AND FUNCTIONAL-PROPERTIES OF IN-VITRO OXIDIZED LOW-DENSITY-LIPOPROTEIN
Autore:
HARDUIN P; TAILLEUX A; LESTAVEL S; CLAVEY V; FRUCHART JC; FIEVET C;
Indirizzi:
INST PASTEUR,SERLIA,INSERM,U325,1 RUE PROF CALMETTE F-59019 LILLE FRANCE INST PASTEUR,SERLIA,INSERM,U325 F-59019 LILLE FRANCE FAC PHARM LILLE F-59045 LILLE FRANCE
Titolo Testata:
Journal of lipid research
fascicolo: 5, volume: 36, anno: 1995,
pagine: 919 - 930
SICI:
0022-2275(1995)36:5<919:IAFOIO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONOCYTE-MACROPHAGES; APOLIPOPROTEIN-B EPITOPE; MONOCLONAL-ANTIBODIES; ATHEROSCLEROTIC LESIONS; OXIDATIVE MODIFICATION; RECEPTOR INTERACTION; SCAVENGER RECEPTOR; APO-B; LDL; EXPRESSION;
Keywords:
COPPER IONS; PHOSPHOLIPASE A(2); LIPOXYGENASE; APO-B-100 EPITOPES; APOB/E RECEPTOR; MACROPHAGIC SCAVENGER RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
P. Harduin et al., "IMMUNOLOGICAL AND FUNCTIONAL-PROPERTIES OF IN-VITRO OXIDIZED LOW-DENSITY-LIPOPROTEIN", Journal of lipid research, 36(5), 1995, pp. 919-930

Abstract

We studied the effect of in vitro moderate oxidization on low densitylipoprotein (LDL) conformation and metabolism. LDL was modified with either copper ions or phospholipase A(2) plus lipoxygenase and, in both cases, mild oxidative conditions were used. The resulting conformational changes were investigated by studying immunological and biological properties of oxidized LDL. The immunoreactivity of apolipoprotein (ape) B-100 was examined using seven monoclonal antibodies. The biological implications of conformational changes were provided by cell-lipoprotein interaction studies using human fibroblasts and mouse peritoneal macrophages. Enzymatically treated LDL presented a relatively less oxidative degree of modification because it generated lower levels of TBARS, and displayed a lower electronegativity and more comparable cellular interactions with those of native LDL. Nevertheless, dramatic immunological changes were measured on both forms of LDL, i.e., a significant increase in the immunoreactivity of an epitope located in the B/Ereceptor binding domain, but also at epitopes far from this site and located in the N-terminal part of the apoB-100 molecule. The immunoreactivity of the C-terminal region was, in contrast, decreased. Yet, as compared with enzymatically oxidized LDL, much more dramatic structural changes with chemically modified LDL were observed, resulting in such a particular conformation of lipoprotein that its interaction with the macrophagic scavenger receptor was favored, but its recognition by the B/E receptor of fibroblasts was abolished. In contrast, despite a lower interaction between enzymatically modified LDL and the B/E receptor, the metabolism of this lipoprotein was quite comparable with thatof native LDL and its degradation with cultured macrophages was poor. The use of in vitro models is common for study of the relationship between oxidized LDL and atherogenesis in humans. The choice of the moreappropriate way to modify lipoproteins is of interest and is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:08:25