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Titolo:
METHANE-INDUCED HEMOLYSIS OF HUMAN ERYTHROCYTES
Autore:
BATLIWALA H; SOMASUNDARAM T; UZGIRIS EE; MAKOWSKI L;
Indirizzi:
FLORIDA STATE UNIV,INST MOLEC BIOPHYS TALLAHASSEE FL 32306 FLORIDA STATE UNIV,INST MOLEC BIOPHYS TALLAHASSEE FL 32306 BOSTON UNIV,DEPT PHYS BOSTON MA 02215 GE CO,CTR RES & DEV SCHENECTADY NY 12301
Titolo Testata:
Biochemical journal
, volume: 307, anno: 1995,
parte:, 2
pagine: 433 - 438
SICI:
0264-6021(1995)307:<433:MHOHE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS SUPERSATURATION TOLERANCES; HYDROSTATIC-PRESSURE; LIPID BILAYERS; PROTEINS; MEMBRANE; BIOMOLECULES; AMPHIPHILES; HEMOLYSIS; ETHANOL; HEXANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
H. Batliwala et al., "METHANE-INDUCED HEMOLYSIS OF HUMAN ERYTHROCYTES", Biochemical journal, 307, 1995, pp. 433-438

Abstract

Human erythrocytes were exposed to high concentrations of methane andnitrogen through the application of elevated partial pressures of these gas molecules. Cell leakage (haemolysis) was measured for cells exposed to these gases under a wide range of experimental conditions. Application of methane produces haemolysis at pressures far below the hydrostatic pressures known to disrupt membrane or protein structure. Theeffects of changes in buffer, temperature, diffusion rate and detergents were studied. Methane acts co-operatively with detergents to produce haemolysis at much lower detergent concentration than is required in the absence of methane or in the presence of nitrogen. At sufficiently high concentrations of methane, all cells are haemolysed. Increasedtemperature enhances the effect. Methane produces 50% haemolysis at aconcentration of about 0.33 M compared with about 7.5 M methanol required for the same degree of haemolysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:48:30