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Titolo:
CARBOHYDRATES MEDIATE THE ADHERENCE OF HAMSTER SPERM TO OVIDUCTAL EPITHELIUM
Autore:
DEMOTT RP; LEFEBVRE R; SUAREZ SS;
Indirizzi:
UNIV FLORIDA,CTR ENVIRONM & HUMAN TOXICOL,DEPT PHYSIOL SCI,1 PROGRESSBLVD ALACHUA FL 32615
Titolo Testata:
Biology of reproduction
fascicolo: 6, volume: 52, anno: 1995,
pagine: 1395 - 1403
SICI:
0006-3363(1995)52:6<1395:CMTAOH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONA-PELLUCIDA; MOUSE SPERM; INVITRO CAPACITATION; ACROSOME REACTION; CELL-ADHESION; IN-VITRO; EPIDIDYMAL FLUID; CAUDAL ISTHMUS; BOVINE SPERM; LIMAX-FLAVUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Demott et al., "CARBOHYDRATES MEDIATE THE ADHERENCE OF HAMSTER SPERM TO OVIDUCTAL EPITHELIUM", Biology of reproduction, 52(6), 1995, pp. 1395-1403

Abstract

The lower isthmus of the mammalian oviduct appears to serve as a reservoir for sperm that are retained by adherence to the epithelium. By inhibiting sperm binding within excised hamster oviducts and making useof carbohydrate probes, we have characterized the adherence of sperm in the reservoir and established a potential biochemical mechanism forthe adherence and release of sperm. Fetuin and its terminal sugar, N-acetylneuraminic acid, interfered with the adherence of sperm to the oviductal epithelium. Labeled fetuin bound to the acrosomal region of fresh epididymal sperm, but not hyperactivated sperm, which have previously been reported to release from epithelial binding. Western blots labeled with fetuin and sialic acid-recognizing lectins identified proteins at several molecular masses that are candidates for the sperm surface component involved in adherence. Labeling of some of these candidates was reduced in samples from hyperactivated sperm. These results indicate that a sialylated oligosaccharide similar to that found on fetuin may be recognized by a sperm surface component and mediate bindingto the oviductal epithelium. Release may be accomplished by loss or modification of the component during capacitation or hyperactivation.

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Documento generato il 28/11/20 alle ore 03:50:55