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Titolo:
FUNCTIONAL SYNERGISM IN THE CARBOHYDRATE-INDUCED ACTIVATION OF LIVER-TYPE PYRUVATE-KINASE GENE-EXPRESSION
Autore:
LIU ZR; TOWLE HC;
Indirizzi:
UNIV MINNESOTA,DEPT BIOCHEM,4-225 MILLARD HALL MINNEAPOLIS MN 55455 UNIV MINNESOTA,DEPT BIOCHEM MINNEAPOLIS MN 55455 UNIV MINNESOTA,INST HUMAN GENET MINNEAPOLIS MN 55455
Titolo Testata:
Biochemical journal
, volume: 308, anno: 1995,
parte:, 1
pagine: 105 - 111
SICI:
0264-6021(1995)308:<105:FSITCA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; RAT-LIVER; THYROID-HORMONE; RESPONSE ELEMENT; GAL4 DERIVATIVES; PRIMARY CULTURE; COMMON FACTOR; S14 GENE; PROMOTER; GLUCOSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
Z.R. Liu e H.C. Towle, "FUNCTIONAL SYNERGISM IN THE CARBOHYDRATE-INDUCED ACTIVATION OF LIVER-TYPE PYRUVATE-KINASE GENE-EXPRESSION", Biochemical journal, 308, 1995, pp. 105-111

Abstract

Hepatic expression of the liver-type pyruvate kinase (L-PK) gene is induced at the transcriptional level by increased carbohydrate metabolism in the rat. The carbohydrate response of the L-PK gene requires sequences from -171 to -124, which encompass adjacent major late transcription factor (MLTF)-like and hepatic nuclear factor (HNF)-4 binding sites, Neither site alone is capable of conferring a response, promptingus to explore the mechanism of synergy between the MLTF-like factor and HNF-4. Spacing requirements between the two factor binding sites were tested by generating a series of mutations that altered the distance between these sites, Surprisingly, all of the constructs with spacing mutations were capable of responding to elevated glucose when introduced into primary hepatocytes. Thus the glucose response does not depend on the rigid phasing of the MLTF-like and HNF-4 factors, suggestingthat the factors binding to these two sites do not interact directly with each other. Substitution or inversion of the PK HNF-4 site abrogated the response to glucose and also significantly suppressed the promoter activity under non-inducing conditions. We conclude that the MLTFlike factor and HNF-4 co-operate functionally to maintain the basal activity, as well as the carbohydrate responsiveness, of the L-PK gene,A mechanism other than co-operative DNA binding is responsible for the synergism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:49:05