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Titolo:
MODIFICATION OF HUMAN GLIOMA LOCOMOTION IN-VITRO BY CYTOKINES EGF, BFGF, PDGFBB, NGF, AND TNF-ALPHA
Autore:
CHICOINE MR; MADSEN CL; SILBERGELD DL;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT NEUROL SURG,CAMPUS BOX 8057,660 S EUCLIDAVE ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT NEUROL SURG ST LOUIS MO 63110
Titolo Testata:
Neurosurgery
fascicolo: 6, volume: 36, anno: 1995,
pagine: 1165 - 1170
SICI:
0148-396X(1995)36:6<1165:MOHGLI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; MALIGNANT HUMAN GLIOMAS; CENTRAL-NERVOUS-SYSTEM; FACTOR RECEPTOR GENE; HUMAN GLIOBLASTOMA; CELL-LINES; TRANSFORMED-CELLS; B-CHAIN; EXPRESSION; AMPLIFICATION;
Keywords:
BRAIN TUMOR; CELL CULTURE; CELL LOCOMOTION; CYTOKINES; GLIOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M.R. Chicoine et al., "MODIFICATION OF HUMAN GLIOMA LOCOMOTION IN-VITRO BY CYTOKINES EGF, BFGF, PDGFBB, NGF, AND TNF-ALPHA", Neurosurgery, 36(6), 1995, pp. 1165-1170

Abstract

CYTOKINES EXERT RECEPTOR-MEDIATED control over glia. Up-regulation ofreceptor expression or cytokine production corresponds with the acquisition of a neoplastic phenotype. A modified radial dish assay was used to determine whether in vitro locomotion of glioma cells is modifiedby the epidermal growth factor, the basic fibroblast growth factor, the bb dimer of platelet-derived growth factor, the nerve growth factor, or the tumor necrosis factor alpha. Human glioma cells were plated in the center of a petri dish with one of these cytokines in 0.5 mi agar (50 ng/ml if the cytokine was distributed evenly throughout the dish) at one edge, and 0.5 mi plain agar at the opposite edge. After 24 hours, a central zone of cells was established; the agar was gelatinized. Feeding medium was added to the dish, and slow elution from the agarestablished a cytokine gradient. Cell counts were performed daily over 6 to 10 days at predetermined distances on both sides of the centralzone to assess directional cellular movement with respect to the cytokine gradient and the plain agar. The epidermal growth factor caused continuous chemoattraction, whereas the tumor necrosis factor alpha caused slight chemorepulsion for 24 to 48 hours, followed by strong chemoattraction. The bb dimer of platelet-derived growth factor, the basic fibroblast growth factor, and the nerve growth factor all maintained chemorepulsion over the entire 6 to 10 days. Therefore, the cytokines did affect glioma cell motility in vitro, and the modified radial dish assay used in this study provided a useful in vitro model for assessing the impact of the cytokines on glioma cell locomotion. Further understanding of this phenomenon might facilitate the development of therapies to limit glioma cell locomotion and invasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:59:29