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Titolo:
PERFORMANCE OF A SERIES OF NOVEL N-SUBSTITUTED ACRYLAMIDES IN CAPILLARY ELECTROPHORESIS OF DNA FRAGMENTS
Autore:
SIMOALFONSO E; GELFI C; LUCISANO M; RIGHETTI PG;
Indirizzi:
LITA,VIA FRATELLI CERVI 93 I-20090 SEGRATE MILANO ITALY UNIV VALENCIA,FAC QUIM,DEPT QUIM ANALIT E-46100 BURJASSOT SPAIN CNR,ITBA I-20133 MILAN ITALY UNIV MILAN,DIP SCI & TECNOL ALIMENTARI & MICROBIOL I-20133 MILAN ITALY UNIV CALABRIA,DEPT CELL BIOL I-87036 COSENZA ITALY
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 756, anno: 1996,
pagine: 255 - 261
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACRYLOYL TRIS GELS; ARRAY ELECTROPHORESIS; ZONE ELECTROPHORESIS; HIGH-SPEED; RESTRICTION FRAGMENTS; CHIPS; POLYACRYLAMIDE; SEPARATION;
Keywords:
ACRYLAMIDES, N-SUBSTITUTED; N-ACRYLOYLAMINOPROPANOL; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
E. Simoalfonso et al., "PERFORMANCE OF A SERIES OF NOVEL N-SUBSTITUTED ACRYLAMIDES IN CAPILLARY ELECTROPHORESIS OF DNA FRAGMENTS", Journal of chromatography, 756(1-2), 1996, pp. 255-261

Abstract

DNA separations by capillary electrophoresis in viscous solutions of novel polymers, made with Omega-hydroxyl, N-substituted acrylamides (notably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB) are evaluated. Whereas in standard poly(acrylamide), at 6% concentration, the theoretical plate number (N) does not exceed 500 000, in 6%poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1200 000 are obtained. Also, copolymers of AAP and AAB give N values in excess of 1 million plates. The two novel monomers (AAP and AAB) remain extremely stable during alkaline hydrolysis and display very good hydrophilicity, while being devoid of the noxious habit of autopolymerization and auto-reticulation exhibited by the previous monomer of this series (N-acryloyl amino ethoxy ethanol). The reasons for such a good performance of the Omega-substituted acrylamide derivatives could bethat their polymers may form hydrogen bonds via their distal -OH group during DNA separation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 15:35:43