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Titolo:
MACAQUE SPERM FUSION WITH ZONA-FREE HAMSTER OOCYTES
Autore:
VANDEVOORT CA; OVERSTREET JW;
Indirizzi:
UNIV CALIF DAVIS,CALIF REG PRIMATE RES CTR DAVIS CA 95616 UNIV CALIF DAVIS,DIV REPROD BIOL & MED,DEPT OB GYN DAVIS CA 95616
Titolo Testata:
Journal of medical primatology
fascicolo: 5, volume: 25, anno: 1996,
pagine: 356 - 360
SICI:
0047-2565(1996)25:5<356:MSFWZH>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; MACACA-FASCICULARIS; HUMAN-SPERMATOZOA; CELL-ADHESION; EGG FUSION; PELLUCIDA; FERTILIZATION; PENETRATION; INVITRO; PROTEIN;
Keywords:
MACACA FASCICULARIS; SEMEN; CAPACITATION; ACROSOME REACTION; FERTILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Vandevoort e J.W. Overstreet, "MACAQUE SPERM FUSION WITH ZONA-FREE HAMSTER OOCYTES", Journal of medical primatology, 25(5), 1996, pp. 356-360

Abstract

Semen from six cynomolgus macaques was washed twice by centrifugationin BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, thenwashed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophore-treated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 18:54:04