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Titolo:
COMPARISON OF 4 DNA-BASED METHODS FOR STRAIN DELINEATION OF CANDIDA-LUSITANIAE
Autore:
KING D; RHINECHALBERG J; PFALLER MA; MOSER SA; MERZ WG;
Indirizzi:
JOHNS HOPKINS UNIV HOSP,DEPT PATHOL,MEYER B1-193 BALTIMORE MD 21287 JOHNS HOPKINS MED INST,DEPT PATHOL BALTIMORE MD 21287 OREGON HLTH SCI UNIV PORTLAND OR 97201 UNIV ALABAMA,SCH MED BIRMINGHAM AL 35233
Titolo Testata:
Journal of clinical microbiology
fascicolo: 6, volume: 33, anno: 1995,
pagine: 1467 - 1470
SICI:
0095-1137(1995)33:6<1467:CO4DMF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; AMPHOTERICIN-B; OPPORTUNISTIC PATHOGEN; HISTOPLASMA-CAPSULATUM; IDENTIFICATION; EPIDEMIOLOGY; RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
D. King et al., "COMPARISON OF 4 DNA-BASED METHODS FOR STRAIN DELINEATION OF CANDIDA-LUSITANIAE", Journal of clinical microbiology, 33(6), 1995, pp. 1467-1470

Abstract

Four methods for the accurate delineation of epidemiologically related and unrelated strains of Candida lusitaniae were compared, Three pulsed-field electrophoretic methods, including two contour-clamped homogeneous field gel electrophoresis methods (EKP-1 and EKP-2) yielding electrophoretic karyotype patterns of intact chromosomal DNA and a method in which the chromosomal DNA was macrodigested with the endonucleaseSfiI prior to pulsed-field electrophoresis (MDP), and a random amplified polymorphic DNA (RAPD) assay were evaluated, A selected panel of 21 well-characterized isolates representing 13 strains of C. lusitaniae, including 7 epidemiologically related isolates of one strain (group I-A), 3 epidemiologically related isolates of another strain (group I-B), and 11 epidemiologically unrelated isolates (group II), were tested, All isolates were coded and tested in a blinded manner, All seven group I-A isolates were confirmed to be a single strain by the EKP-1 and MDP methods, and the three group I-B isolates were shown to de a single strain by the EKP-1, EKP-2, MDP, and RAPD methods, Subtle differences were noted with two of the group I-A isolates by the EKP-2 method,whereas three of these isolates were different by the RAPD method, Each group II isolate had distinct patterns by all four methods, These data support the fact that the three pulsed-field electrophoretic methods and the RAPD) method can be used to delineate strains of C. lusitaniae. The EKP-1, EKP-2, and MDP gave results that correlated with the epidemiologic characteristics of the isolates tested in the study, whereas the RAPD method was perhaps too sensitive in detecting DNA changesfor epidemiologic studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 14:19:03