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Titolo:
CHARACTERIZATION OF [HYDROXYPROLINE(9)]LUTEINIZING HORMONE-RELEASING HORMONE AND ITS SMALLEST PRECURSOR FORMS IN IMMORTALIZED LUTEINIZING-HORMONE-RELEASING HORMONE-SECRETING NEURONS (GT1-7), AND EVALUATION OF THEIR MODE OF ACTION ON PITUITARY-CELLS
Autore:
GAUTRON JP; POULIN B; KORDON C; DROUVA SV;
Indirizzi:
UNIV AIX MARSEILLE 2,FAC MED,ICNE,CNRS,UMR 9941,BLVD PIERRE DRAMARD F-13916 MARSEILLE 20 FRANCE CTR PAUL BROCA,INSERM,U159,UNITE DYNAM SYST NEUROENDOCRINIENS F-75014PARIS FRANCE
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1-2, volume: 110, anno: 1995,
pagine: 161 - 173
SICI:
0303-7207(1995)110:1-2<161:CO[HH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID RELEASE; C-TERMINAL FRAGMENTS; PROTEIN-KINASE-C; HYPOTHALAMIC NEURONS; GONADOTROPE LINEAGE; SEXUAL RECEPTIVITY; FEMALE RAT; LHRH; FACILITATION; INVITRO;
Keywords:
LUTEINIZING HORMONE-RELEASING HORMONE; [HYDROXYPROLINE(9)]LUTEINIZING HORMONE-RELEASING HORMONE; FREE ACID DECAPEPTIDES; [HYDROXYPROLINEG]LUTEINIZING HORMONE-RELEASING HORMONE- AND LUTEINIZING HORMONE-RELEASING HORMONE-PRECURSORS; COUPLING MECHANISM(S); ALPHA-T3-1 CELLS; PLC; PLA(2);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
J.P. Gautron et al., "CHARACTERIZATION OF [HYDROXYPROLINE(9)]LUTEINIZING HORMONE-RELEASING HORMONE AND ITS SMALLEST PRECURSOR FORMS IN IMMORTALIZED LUTEINIZING-HORMONE-RELEASING HORMONE-SECRETING NEURONS (GT1-7), AND EVALUATION OF THEIR MODE OF ACTION ON PITUITARY-CELLS", Molecular and cellular endocrinology, 110(1-2), 1995, pp. 161-173

Abstract

[Hydroxyproline(9)]luteinizing hormone-releasing hormone ([Hyp(9)]LHRH), an endogenous hydroxylated post-translational product of the LHRH sequence, has been isolated from mammalian hypothalamus. Using the LHRH-hypothalamic cell line (GT1-7) of fetal origin, we attempted to define the substrates available for the hydroxylation process during LHRH synthesis and to characterize immunologically the [Hyp(9)]LHRH and pro-[Hyp(9)]LHRH forms with anti-LHRH antibodies of different specificities after separation by HPLC. Their biological activity and mode of action were evaluated and compared to that of LHRH and LHRH intermediate precursors in normal pituitary cells and in a gonanodotrope cell line alpha T3-1. LHRH-like immunoreactivity was progressively increased in cells and media during cell culture. [Hyp(9)]LHRH and its two smallestprecursor forms ([Hyp(9)]LHRH-(Gly(11)) and -(11-13)) were detected in cells and in media. They were;simultaneously detected with the homologous LHRH molecular forms indicating that the hydroxylation occurs early in the processing of pro-LHRH. [Hyp(9)]LHRH-like molecules were more abundant than LHRH forms in media. This predominant release may thus represent a physiological process occurring during fetal life. Free acid forms of both decapeptides were detected only in cells. Furthermore, the results obtained suggest that conversion of Gln(1) in pyroGlu(1) occurs before or during processing into the hydroxylated or non-hydroxylated LHRH intermediate (11-13)-precursors. The biosynthetic pathway is thus common for both decapeptides and it is not altered by the hydroxylation process. LHRH and [Hyp(9)]LHRH shared the same receptor for their biological activity, as assessed by measuring luteinizing hormone release and activation of phospholipase C and A(2). [Hyp(9)]LHRH was, however, less potent than LHRH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 20:59:52