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Titolo:
STRETCH-ACTIVATED CHANNEL BLOCKERS MODULATE CELL-VOLUME IN CARDIAC VENTRICULAR MYOCYTES
Autore:
SULEYMANIAN MA; CLEMO HF; COHEN NM; BAUMGARTEN CM;
Indirizzi:
VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL RICHMOND VA23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHYSIOL RICHMOND VA23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT INTERNAL MED CARDIOL RICHMOND VA 23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT SURG RICHMOND VA 23298 ARMENIAN ACAD SCI,DEPT BIOPHYS YEREVAN 375044 ARMENIA
Titolo Testata:
Journal of Molecular and Cellular Cardiology
fascicolo: 1, volume: 27, anno: 1995,
pagine: 721 - 728
SICI:
0022-2828(1995)27:1<721:SCBMCI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURED CHICK HEART; MECHANOSENSITIVE ION CHANNELS; CHLORIDE CONDUCTANCE; K+ CHANNEL; ARRHYTHMIAS; GADOLINIUM; OOCYTES; ANION;
Keywords:
CELL VOLUME; MECHANOELECTRICAL FEEDBACK; GADOLINIUM; 9-ANTHRACENE CARBOXYLIC ACID; ATP-DEPENDENT POTASSIUM CHANNELS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Suleymanian et al., "STRETCH-ACTIVATED CHANNEL BLOCKERS MODULATE CELL-VOLUME IN CARDIAC VENTRICULAR MYOCYTES", Journal of Molecular and Cellular Cardiology, 27(1), 1995, pp. 721-728

Abstract

Stretch-activated channels (SAC) are postulated to regulate cell volume. While this hypothesis is appealing, direct evidence is lacking. Using digital video microscopy, we found that pharmacological blockade of SACs alters the cell volume of isolated rabbit ventricular myocytes during hypoosmotic stress, Under control conditions, relative cell volume increased from 1.0 to 1.311 +/- 0.019 after 10 min in 195 mosmol/lsolution, The cation SAC blocker gadolinium (Gd3+; 10 mu M) reduced the amount of swelling in hypoosmotic solution by 24% and induced a regulatory volume decrease otherwise not observed, In contrast, the anionSAC blocker 9-anthracene carboxylic acid (9-AC; 1 mM) increased swelling by 44% under the same conditions, Based on the direction of SAC currents, Gd3+ and 9-AC are expected to have opposite effects on cell volume. Furthermore, Gd3+ and 9-AC changed cell volume by only similar to 2% in isosmotic solutions when SACs are expected to be closed. This supports the idea that Gd3+ and 9-AC affect stretch-activated transport processes, In contrast, omitting bath Ca2+ did not alter cell volumeunder iso- or hypoosmotic conditions suggesting stretch-activated Ca2 influx is not important in setting cell volume, Not all channels canaffect cell volume. Opening ATP-sensitive K+ channels with aprikalim (100 mu M) or blocking them with glibenclamide (1 mu M) did not alter cell volume under isosmotic or hypoosmotic conditions, These data support the idea that SACs are involved in cardiac cell volume regulation.

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Documento generato il 20/09/20 alle ore 03:46:58