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Titolo:
A RAPID, HIGH-CAPACITY ASSAY FOR BASIC FIBROBLAST GROWTH-FACTOR BINDING
Autore:
HERBLIN WF; BARBERA FA;
Indirizzi:
DUPONT MERCK PHARMACEUT CO,CARDIOVASC DIS RES WILMINGTON DE 19880 DUPONT MERCK PHARMACEUT CO,CARDIOVASC DIS RES WILMINGTON DE 19880
Titolo Testata:
Life sciences
fascicolo: 15, volume: 56, anno: 1995,
pagine: 1257 - 1264
SICI:
0024-3205(1995)56:15<1257:ARHAFB>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; LOW AFFINITY BINDING; TUMOR-GROWTH; PENTOSAN POLYSULFATE; DNA-SYNTHESIS; SITES; CELLS; ANGIOGENESIS; MODULATION; MIGRATION;
Keywords:
BASIC FIBROBLAST GROWTH FACTOR; SURAMIN; DIMERCAPTOTHIADIAZOLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
W.F. Herblin e F.A. Barbera, "A RAPID, HIGH-CAPACITY ASSAY FOR BASIC FIBROBLAST GROWTH-FACTOR BINDING", Life sciences, 56(15), 1995, pp. 1257-1264

Abstract

A rapid, high capacity assay for the binding of basic fibroblast growth factor has been developed. Rat lung tissue was selected as the optimum source of membranes and 2M sodium chloride used to remove endogenous growth factor. The assay has been adapted to the Millipore Multi-Screen system so that it can be run in 96-well format with a volume of 300 mu l. The assay has been validated through the demonstration of inhibition by standard inhibitors such as suramin and protamine sulfate. The assay has proven useful for the screening of random compounds as well as the more detailed examination of suspected inhibitors. By running compounds in the presence and absence of a mid-range concentration of unlabeled bFGF, an estimate of the proportion of the inhibition dueto high affinity binding can be obtained. Suramin and protamine sulfate show no selectivity and inhibit high affinity binding and overall binding with similar potencies. Another inhibitor, dimercaptothiadiazole, is more potent against high affinity binding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 11:03:05