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Titolo:
ENHANCEMENT OF HUMAN HEMATOPOIESIS BY MAST-CELL GROWTH-FACTOR IN HUMAN-SHEEP CHIMERAS CREATED BY THE IN-UTERO TRANSPLANTATION OF HUMAN FETAL HEMATOPOIETIC-CELLS
Autore:
FLAKE AW; HENDRICK MH; RICE HE; TAVASSOLI M; ZANJANI ED;
Indirizzi:
VET ADM MED CTR,1000 LOCUST ST RENO NV 89520 UNIV CALIF SAN FRANCISCO,DEPT SURG SAN FRANCISCO CA 94143 DEPT VET AFFAIRS MED CTR JACKSON MS 39216 UNIV NEVADA,SCH MED,DEPT VET AFFAIRS MED CTR RENO NV 89557
Titolo Testata:
Experimental hematology
fascicolo: 3, volume: 23, anno: 1995,
pagine: 252 - 257
SICI:
0301-472X(1995)23:3<252:EOHHBM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT RECEPTOR; COLONY-STIMULATING FACTOR; SL-SLD MICE; STEM-CELLS; PROGENITOR CELLS; SI-LOCUS; W-LOCUS; LIGAND; MOUSE; ENGRAFTMENT;
Keywords:
IN UTERO TRANSPLANTATION; HUMAN-SHEEP CHIMERAS; MAST CELL GROWTH FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
A.W. Flake et al., "ENHANCEMENT OF HUMAN HEMATOPOIESIS BY MAST-CELL GROWTH-FACTOR IN HUMAN-SHEEP CHIMERAS CREATED BY THE IN-UTERO TRANSPLANTATION OF HUMAN FETAL HEMATOPOIETIC-CELLS", Experimental hematology, 23(3), 1995, pp. 252-257

Abstract

We have previously described a unique model of long-term, multilineage, human hematopoietic chimerism in sheep created by the in utero transplantation of human hematopoietic stem cells (HSC) into pre-immune fetal lambs. In this study, we examined the effect of chronic administration of recombinant human mast cell growth factor (rhMGF) on 1) human cell engraftment in pre-immune sheep and 2) human cell expression in human-sheep chimeras at 2-years posttransplant. rhMGF (25 mu g/kg) or saline was administered in utero via chronic intraperitoneal (IP) catheters to three separate sets of twin fetuses on alternate days for 10 doses following transplantation of human HSC. Flow-cytometric and karyotype analyses of peripheral blood from two sets of twins at 45-days posttransplant and of peripheral blood from the remaining set of twins at birth revealed a significant increase in percentages of donor (human) progenitors and cells in rhMGF-treated lambs. rhMGF (60 mu g/kg/day)was also administered by IP injection to two, 2 year-old, human-sheepchimeras for 18 consecutive days. Flow-cytometric analysis of peripheral blood and bone marrow revealed a six- to seven-fold increase in human cell expression. The effect on early human progenitors (i.e., colony-forming unit-mix [CFU-Mix], CFU granulocyte/macrophage [CFU-GM], and burst-forming unit-erythroid [BFU-E]) was determined by karyotype analysis of individual colonies grown under conditions favoring human cell growth. A three- to five-fold increase in human CFU-Mix and BFU-E occurred with a minimal increase in CFU-GM. This in vivo study supportsin vitro data suggesting that MGF is a powerful regulator of human hematopoiesis and preferentially stimulates early hematopoietic progenitors. It also supports the potential value of the human-sheep model forthe in vivo study of normal and abnormal human hematopoiesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:00:51