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Titolo:
IN-VITRO SEPARATION AND EXPANSION OF CD4 LYMPHOCYTES FROM HIV-INFECTED INDIVIDUALS WITHOUT ACTIVATION OF HIV-INFECTION
Autore:
NIELSEN SD; NIELSEN JO; HANSEN JES;
Indirizzi:
HVIDOVRE UNIV HOSP,INFECT DIS LAB,DEPT 144 DK-2650 HVIDOVRE DENMARK HVIDOVRE UNIV HOSP,INFECT DIS LAB,DEPT 144 DK-2650 HVIDOVRE DENMARK
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 200, anno: 1997,
pagine: 107 - 112
SICI:
0022-1759(1997)200:1-2<107:ISAEOC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELLS; IMMUNODEFICIENCY; INHIBITION; INVITRO; HELPER;
Keywords:
CD4; HIV; MAGNETIC CELL SEPARATION; EXPANSION; VIROLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
S.D. Nielsen et al., "IN-VITRO SEPARATION AND EXPANSION OF CD4 LYMPHOCYTES FROM HIV-INFECTED INDIVIDUALS WITHOUT ACTIVATION OF HIV-INFECTION", Journal of immunological methods, 200(1-2), 1997, pp. 107-112

Abstract

In order to offer a gene therapy-based treatment against AIDS, it is likely to be necessary to harvest and culture CD4 cells from HIV-positive patients without activating the HIV infection. We have used a magnetic cell sorting (MACS) system to enrich CD4 cells. Using positive selection, CD4 cells from a total of 14 patients were enriched from a mean percentage of CD4 cells in PBMC of 18% to 91% CD4 cells in the enriched cell fraction. Furthermore, we found that this separation did notlead to an increase in viral load. The MACS performed equally well oncells from HIV-positive patients and HIV-negative donors. CD4 cells from HIV-positive patients were readily expanded with PHA; 19-fold by day 10, 50-fold by day 20, and 156-fold by day 25. However, CD4 cells from HIV-positive patients grew at a slower rate than CD4 cells from HIV-negative donors. The expanded CD4 cells showed a high degree of CD4 expression and no loss of polyclonality. Only in two of six cultures were we able to detect HIV-antigen production, and using an LTR-PCR andan RT assay, we did not find activation of the HIV infection during the culture period. Thus, the method described separates and expands CD4 cells from HIV-positive patients without activation of the HIV infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 06:37:49