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Titolo:
FUNCTIONAL ASSAYS FOR EVALUATION OF SEROGROUP-B MENINGOCOCCAL STRUCTURES AS MEDIATORS OF HUMAN OPSONOPHAGOCYTOSIS
Autore:
LEHMANN AK; HALSTENSEN A; HOLST J; BASSOE CF;
Indirizzi:
UNIV BERGEN,DEPT MED B,HAUKELAND HOSP N-5021 BERGEN NORWAY NATL INST PUBL HLTH,DEPT VACCINOL N-0462 OSLO NORWAY
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 200, anno: 1997,
pagine: 55 - 68
SICI:
0022-1759(1997)200:1-2<55:FAFEOS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE VESICLE VACCINE; REACTING SERUM OPSONINS; FLOW CYTOMETRIC ASSAY; POLYMORPHONUCLEAR LEUKOCYTES; NEISSERIA-MENINGITIDIS; CHEMI-LUMINESCENCE; HUMAN-NEUTROPHILS; LATEX BEADS; PHAGOCYTOSIS; DISEASE;
Keywords:
PHAGOCYTOSIS; SEROGROUP B MENINGOCOCCUS; MENINGOCOCCAL ANTIGEN; FLOW CYTOMETRY; CHEMILUMINESCENCE; CONFOCAL LASER SCANNING MICROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
A.K. Lehmann et al., "FUNCTIONAL ASSAYS FOR EVALUATION OF SEROGROUP-B MENINGOCOCCAL STRUCTURES AS MEDIATORS OF HUMAN OPSONOPHAGOCYTOSIS", Journal of immunological methods, 200(1-2), 1997, pp. 55-68

Abstract

Functional flow cytometry and chemiluminescence (CL) assays have beenmodified to identify serogroup B meningococcal structures that mediate anti-meningococcal opsonophagocytosis. Serogroup B meningococcal outer membrane vesicles (OMV) were adsorbed to fluorescent latex beads (OMV-beads) and opsonized with acute phase and convalescence sera from patients with serogroup B meningococcal disease. Phagocytosis of these beads by human monocytes and polymorphonuclear leukocytes (non-lymphocytes) was dependent on both antigen exposure on the bead surface and on serum opsonization. OMV-beads opsonized with serum from a patient recovering from meningococcal disease, caused 97% of the non-lymphocytesto phagocytose an average of 15.8 beads per cell with a CL response of 46 550 mVs, whereas opsonized control beads were phagocytosed by 19%of the non-lymphocytes with 1.1 beads per cell and a CL response of 53 mVs. Increased amounts of functional, anti-OMV opsonins were detected during infection, and opsonized OMV-beads elicited phagocyte responses of similar magnitude to those of opsonized whole meningococci. Phagocyte internalization of OMV-beads was confirmed by confocal laser scanning microscopy. We conclude that epitopes on the meningococcal outermembrane are recognized by anti-meningococcal opsonins in these functional phagocytosis assays, which provide a basis for subsequent evaluation of various purified bacterial components as mediators of human opsonophagocytic responses and hence future vaccine constituents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:36:34