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Titolo:
AMPLIFICATION AND DIFFERENTIAL EXPRESSION OF MEMBERS OF THE ERBB-GENEFAMILY IN HUMAN GLIOBLASTOMA
Autore:
SCHLEGEL J; STUMM G; BRANDLE K; MERDES A; MECHTERSHEIMER G; HYNES NE; KIESSLING M;
Indirizzi:
UNIV KLIN REGENSBURG,INST PATHOL D-93042 REGENSBURG GERMANY UNIV REGENSBURG,INST PATHOL W-8400 REGENSBURG GERMANY UNIV HEIDELBERG,INST NEUROPATHOL HEIDELBERG GERMANY UNIV HEIDELBERG,INST PATHOL HEIDELBERG GERMANY FRIEDRICH MIESCHER INST CH-4002 BASEL SWITZERLAND
Titolo Testata:
Journal of neuro-oncology
fascicolo: 3, volume: 22, anno: 1994,
pagine: 201 - 207
SICI:
0167-594X(1994)22:3<201:AADEOM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; TYROSINE KINASE-ACTIVITY; EGF RECEPTOR; PROTEIN; OVEREXPRESSION; TUMORS; SEQUENCES; ONCOGENE; BINDING; GLIOMAS;
Keywords:
MALIGNANT GLIOMAS; ONCOGENES; GROWTH FACTORS; GENE AMPLIFICATION; EGF RECEPTOR; ERBB2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J. Schlegel et al., "AMPLIFICATION AND DIFFERENTIAL EXPRESSION OF MEMBERS OF THE ERBB-GENEFAMILY IN HUMAN GLIOBLASTOMA", Journal of neuro-oncology, 22(3), 1994, pp. 201-207

Abstract

The objective of the present study was to determine the frequency of amplifications of three different members of the erbB gene family in human glioblastoma multiforme (GEM). We investigated 47 glial tumors (37 GEM WHO grade IV, 5 anaplastic astrocytomas WHO III and 5 astrocytomas WHO II) by Southern and Western analysis, and immunocytochemistry. Gene amplification of erbB genes in human malignant gliomas was restricted to the EGF receptor (EGFR) gene, erbB-1. We found amplification of the EGFR gene in 49% (18/37) of GEM but not in the astrocytomas WHO II/III. The erbB-2 and erbB-3 genes showed no amplification in the tumor specimens investigated in this study. At the protein level we foundoverexpression of the EGF receptor in 86% (32/37) by Western analysisand in 92% (34/37) by immunocytochemistry. Expression of the ERBB2 protein was present in 54% (20/37) but immunoreactivity was much weaker than for EGF receptor and in most cases barely detectable by Western analysis and immunocytochemistry. The ERBB3 protein was not expressed in the glial tumors investigated in this study. Of the three erbB genesonly gene amplification and overexpression of the EGF receptor seems to have an impact on tumor progression of human gliomas. Our data fromimmunohistochemistry indicate that ERBB2 expression in GEM is closelycorrelated with EGF receptor levels and is therefore not useful as anindependent prognostic parameter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 03:30:46