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Titolo:
ELECTROSPRAY MASS-SPECTROMETRY FOR THE CHARACTERIZATION OF THE PURITYOF NATURAL AND MODIFIED OLIGODEOXYNUCLEOTIDES
Autore:
DEROUSSENT A; LECAER JP; ROSSIER J; GOUYETTE A;
Indirizzi:
INST GUSTAVE ROUSSY,CNRS,URA 147,PHYSICOCHIM & PHARMACOL MACROMOLEC BIOL LAB F-94805 VILLEJUIF FRANCE INST ALFRED FESSARD,NEUROPHARMACOL MOLEC LAB F-91191 GIF SUR YVETTE FRANCE
Titolo Testata:
Rapid communications in mass spectrometry
fascicolo: 1, volume: 9, anno: 1995,
pagine: 1 - 4
SICI:
0951-4198(1995)9:1<1:EMFTCO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTISENSE DNA; OLIGONUCLEOTIDES; CHROMATOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
A. Deroussent et al., "ELECTROSPRAY MASS-SPECTROMETRY FOR THE CHARACTERIZATION OF THE PURITYOF NATURAL AND MODIFIED OLIGODEOXYNUCLEOTIDES", Rapid communications in mass spectrometry, 9(1), 1995, pp. 1-4

Abstract

Electrospray ionization mass spectrometry is an accurate and sensitive analytical method to characterize the purity of oligodeoxynucleotides being tested for pharmacological studies. We report the preparation procedure ('desalting') of natural and modified oligodeoxynucleotides (ODNs) and their analysis by negative-ion electrospray mass spectrometry. We evaluate the sensitivity and the accuracy of the method for twoantisense ODN sequences. Mass analysis of the 25-mer phosphorothioatecan be performed to within 0.001% accuracy (standard error of 0.05 Da) for a sample concentration of 12 pmol/mu L. In addition, the adduct ion and the failure sequence can be identified to characterize the antisense ODN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 03:01:23