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Titolo:
ESTABLISHMENT OF A CELL-LINE EXPRESSING HUMAN PARVOVIRUS B19 NONSTRUCTURAL PROTEIN FROM AN INDUCIBLE PROMOTER
Autore:
LERUEZVILLE M; VASSIAS I; PALLIER C; CECILLE A; HAZAN U; MORINET F;
Indirizzi:
HOP ST LOUIS,MICROBIOL SERV,UNITE VIROL,1 AVE CLAUDE VELLEFAUX F-75010 PARIS FRANCE HOP ST LOUIS,MICROBIOL SERV,UNITE VIROL F-75010 PARIS FRANCE CNRS,UPR 9051 F-75010 PARIS FRANCE HOP AVICENNE,LAB BACTERIOL VIROL F-93009 BOBIGNY FRANCE INST COCHIN GENET MOL,INSERM,U380 F-75014 PARIS FRANCE
Titolo Testata:
Journal of General Virology
, volume: 78, anno: 1997,
parte:, 1
pagine: 215 - 219
SICI:
0022-1317(1997)78:<215:EOACEH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTRUCTURAL PROTEIN; VIRUS; TRANSCRIPTION; REPLICATION; GENES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M. Leruezville et al., "ESTABLISHMENT OF A CELL-LINE EXPRESSING HUMAN PARVOVIRUS B19 NONSTRUCTURAL PROTEIN FROM AN INDUCIBLE PROMOTER", Journal of General Virology, 78, 1997, pp. 215-219

Abstract

Human parvovirus B19 non-structural (NS) protein is supposed to play a major role in B19 replication and transcription, and therefore in B19 pathogenicity, Constitutive expression of NS protein in stable cell lines has failed so far, presumably because of its cytotoxicity, To avoid this cytotoxic effect, we have cloned the NS gene in an Epstein-Barr virus episomal vector under the control of a steroid inducible promoter (5xGRE) and transfected this construction into HeLa cells, We obtained stable cell lines inducibly expressing high level of NS protein,with 50% of the cells demonstrating specific nucleo-cytoplasmic staining, In Western blot analysis, three B19 NS proteins (72, 68 and 60 kDa) were found but a unique NS transcript was detected by Northern blotting, The NS protein expressed in HeLa cell lines was demonstrated to be functional as it trans-activates the B19 P6 promoter. These cell lines might be major tools for further study and characterization of B19NS protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 05:25:44