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Titolo:
MOLECULAR-GENETIC ANALYSIS OF THE RESPONSE OF 3 SOIL MICROBIAL COMMUNITIES TO THE APPLICATION OF 2,4-D
Autore:
XIA XQ; BOLLINGER J; OGRAM A;
Indirizzi:
WASHINGTON STATE UNIV,DEPT CROP & SOIL SCI PULLMAN WA 99164 WASHINGTON STATE UNIV,DEPT CROP & SOIL SCI PULLMAN WA 99164
Titolo Testata:
Molecular ecology
fascicolo: 1, volume: 4, anno: 1995,
pagine: 17 - 28
SICI:
0962-1083(1995)4:1<17:MAOTRO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL BACTERIOPLANKTON ASSEMBLAGES; ALCALIGENES-EUTROPHUS JMP134(PJP4); POLYMORPHIC DNA RAPD; 2,4-DICHLOROPHENOXYACETIC ACID; MONOOXYGENASE GENE; HYBRIDIZATION; GLYPHOSATE; BIOMASS; DEGRADATION; CLONING;
Keywords:
COMMUNITY CROSS-HYBRIDIZATION; PESTICIDE AFFECTS; 2,4-D; RAPD FINGERPRINTING; SOIL MICROBIOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
X.Q. Xia et al., "MOLECULAR-GENETIC ANALYSIS OF THE RESPONSE OF 3 SOIL MICROBIAL COMMUNITIES TO THE APPLICATION OF 2,4-D", Molecular ecology, 4(1), 1995, pp. 17-28

Abstract

The responses of three different soil microbial communities to the experimental application of 2,4-dichlorophenoxyacetic acid (2,4-D) were evaluated with a variety of molecular genetic techniques. Two of the three soil communities had histories of prior direct exposure to 2,4-D,and one had no prior direct application of any herbicide. Dominant 2,4-D degrading strains isolated from these soils the previous year werescreened for hybridization with three catabolic genes (tfdA, tfdAII, and tfdB) cloned from the well-studied 2,4-D degradative plasmid, pJP4, revealing varying degrees of similarity with the three genes. Hybridization of total community DNA from the three soils with the tfd gene probes also indicated that pJP4-like tfd genes were not harboured by asignificant percentage of the community. Community level response wasevaluated by the comparison of different treatments by Random Amplified Polymorphic DNA (RAPD) fingerprints and by community DNA cross-hybridization. No differences between treatments within the same soil weredetected in any of the RAPD fingerprints generated with 17 primers. Community DNA cross-hybridization also indicated that the application of 2,4-D at the applied rates did not quantitatively affect the structure of the soil microbial communities present in the three soils duringthe time-frame studied.

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Documento generato il 22/09/20 alle ore 17:24:53