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Titolo:
MOLECULAR-CLONING AND CHARACTERIZATION OF PROFILIN FROM TOBACCO (NICOTIANA-TABACUM) - INCREASED PROFILIN EXPRESSION DURING POLLEN MATURATION
Autore:
MITTERMANN I; SWOBODA I; PIERSON E; ELLER N; KRAFT D; VALENTA R; HEBERLEBORS E;
Indirizzi:
UNIV VIENNA,AKH,INST GEN & EXPTL PATHOL,DIV IMMUNOPATHOL,WAEHRINGER GUERTEL 18 VIENNA AUSTRIA UNIV VIENNA,VIENNA BIOCTR,INST GENET & MICROBIOL A-1010 VIENNA AUSTRIA UNIV MASSACHUSETTS,DEPT BIOL AMHERST MA 01003 UNIV SIENA,DEPT ENVIRONM BIOL I-53100 SIENA ITALY
Titolo Testata:
Plant molecular biology
fascicolo: 1, volume: 27, anno: 1995,
pagine: 137 - 146
SICI:
0167-4412(1995)27:1<137:MACOPF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN-BINDING PROTEIN; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; BIRCH POLLEN; THYONE SPERM; ALLERGEN; TUBES; IDENTIFICATION; CYTOSKELETON; FAMILY; LOCALIZATION;
Keywords:
POLLEN; PROFILIN; TOBACCO; CDNA CLONING; DEVELOPMENTALLY REGULATED EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
I. Mittermann et al., "MOLECULAR-CLONING AND CHARACTERIZATION OF PROFILIN FROM TOBACCO (NICOTIANA-TABACUM) - INCREASED PROFILIN EXPRESSION DURING POLLEN MATURATION", Plant molecular biology, 27(1), 1995, pp. 137-146

Abstract

Profilin has recently been identified as an actin-binding protein in higher plants. A cDNA coding for tobacco profilin, which shared an average sequence identity of 75% with other plant profilins, was isolatedfrom a tobacco pollen cDNA library by antibody screening. Tobacco profilin was expressed in Escherichia coli and purified by affinity to poly-(L-proline) Sepharose. A rabbit antiserum was raised against recombinant tobacco profilin and used to estimate the amount of profilin expressed in different tobacco tissues. Profilin can be detected in different somatic tissues, but the expression is 50-100 fold higher in mature pollen. Immunofluorescence and confocal laser scanning microscopy showed a homogeneous distribution of profilin in the cytoplasm of in vitro cultured pollen grains and pollen tubes of tobacco whereas some growing pollen tubes were stained more intensively a their tip. A possible role of pollen profilin as a developmentally upregulated microfilament precursor in mature pollen is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:08:46