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Titolo:
USE OF GENE AMPLIFICATION TO DETECT CLOSTRIDIUM-DIFFICILE IN CLINICALSPECIMENS
Autore:
RATCLIFF RM; GOODWIN AA; LANSER JA;
Indirizzi:
INST MED & VET SCI,DIV CLIN MICROBIOL,POB 14,RUNDLE MALL ADELAIDE SA 5000 AUSTRALIA
Titolo Testata:
Pathology
fascicolo: 4, volume: 26, anno: 1994,
pagine: 477 - 479
SICI:
0031-3025(1994)26:4<477:UOGATD>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; TOXIN-A; SEQUENCES; COLITIS; DNA;
Keywords:
CLOSTRIDIUM DIFFICILE; TOXINS; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Ratcliff et al., "USE OF GENE AMPLIFICATION TO DETECT CLOSTRIDIUM-DIFFICILE IN CLINICALSPECIMENS", Pathology, 26(4), 1994, pp. 477-479

Abstract

The combined use of an enrichment broth and gene amplification following simple DNA extraction to detect toxigenic strains of Clostridium difficile in feces was investigated by examining feces from 329 cases of suspected C. difficile infection. DNA was extracted by heating the washed centrifuged deposit from the broth in a microwave oven. For comparison, specimens were tested concurrently using standard methods for culture and cytotoxin testing. Amplified fragments were identified by molecular weight estimation, restriction enzyme digestion patterns andSouthern blot hybridization. The combination of an enrichment broth followed by gene amplification was shown to be a sensitive, specific and rapid method for detecting toxigenic strains of C. difficile in feces. Use of the method in diagnostic laboratories may require the development of improved detection and verification systems for the amplifiedgene fragment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 05:57:18