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Titolo:
REPEATED INTRAPORTAL HEPATOCYTE TRANSPLANTATION IN ANALBUMINEMIC RATS
Autore:
ROZGA J; HOLZMAN M; MOSCIONI AD; FUJIOKA H; MORSIANI E; DEMETRIOU AA;
Indirizzi:
CEDARS SINAI MED CTR,DEPT SURG,8700 BEVERLY BLVD,SUITE 8215 LOS ANGELES CA 90048 CEDARS SINAI MED CTR,LIVER SUPPORT UNIT LOS ANGELES CA 90048
Titolo Testata:
Cell transplantation
fascicolo: 2, volume: 4, anno: 1995,
pagine: 237 - 243
SICI:
0963-6897(1995)4:2<237:RIHTIA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; GUNN-RATS; PROTEINS;
Keywords:
HEPATOCYTE; LIVER; TRANSPLANTATION; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
J. Rozga et al., "REPEATED INTRAPORTAL HEPATOCYTE TRANSPLANTATION IN ANALBUMINEMIC RATS", Cell transplantation, 4(2), 1995, pp. 237-243

Abstract

The optimal site for implantation of isolated hepatocytes has not been established. We have developed a novel technique which allows repeated infusion of hepatocytes into the portal system via an indwelling catheter. Seven Nagase Analbuminemic rats (NAR) underwent single intraportal infusion of 2 x 10(7) isolated normal albumin-producing rat hepatocytes. Another seven NAR rats underwent placement of indwelling catheters into the portal venous system via the gastroduodenal vein, Each of them received six batches of 5 x 10(6) normal albumin producing hepatocytes. Seven control NAR rats were infused repeatedly (intraportally) with saline only. Plasma albumin (ELISA) showed significant increasein experimental animals and was more pronounced (p < 0.05) in rats transplanted repeatedly than in those given a single dose of cells. Immunohistochemical staining of the liver sections confirmed the presence of transplanted albumin producing hepatocytes. Rats transplanted with a single large batch of isolated hepatocytes showed liver tissue damage, whereas those subjected to repeated cell infusions had normal liverhistology. We have developed a novel intraportal transplantation method which allows successful engraftment of a large number of isolated hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:58:08