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Titolo:
PRODUCTION OF A BIOLOGICALLY-ACTIVE RECOMBINANT TELEOSTEAN GROWTH-HORMONE IN E-COLI CELLS
Autore:
CHENG CM; LIN CM; SHAMBLOTT M; GONZALEZVILLASENOR LI; POWERS DA; WOODS C; CHEN TT;
Indirizzi:
UNIV MARYLAND,CTR MARINE BIOTECHNOL,600 E LOMBARD ST BALTIMORE MD 21202 UNIV MARYLAND,CTR MARINE BIOTECHNOL BALTIMORE MD 21202 UNIV MARYLAND,DEPT BIOL SCI BALTIMORE MD 21228 JOHNS HOPKINS UNIV,DEPT BIOL BALTIMORE MD 21218 STANFORD UNIV,HOPKINS MARINE STN PACIFIC GROVE CA 93950 UNIV MARYLAND,AGR EXPTL STN COLLEGE PK MD 20742
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1-2, volume: 108, anno: 1995,
pagine: 75 - 85
SICI:
0303-7207(1995)108:1-2<75:POABRT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
I MESSENGER-RNA; NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; RAINBOW-TROUT; MOLECULAR-CLONING; OSMOREGULATORY ACTIONS; TISSUE DISTRIBUTION; COMPLEMENTARY-DNA; PREGROWTH HORMONE; GENE-PRODUCTS;
Keywords:
STRIPED BASS; GROWTH HORMONE; EXPRESSION IN ESCHERICHIA-COLI; RENATURATION; COMPETITIVE RECEPTOR BINDING ASSAY; IGF-I INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
C.M. Cheng et al., "PRODUCTION OF A BIOLOGICALLY-ACTIVE RECOMBINANT TELEOSTEAN GROWTH-HORMONE IN E-COLI CELLS", Molecular and cellular endocrinology, 108(1-2), 1995, pp. 75-85

Abstract

We have isolated and characterized several recombinant lambda phage clones carrying growth hormone (GH) cDNA of striped bass (Morone saxatilis). Nucleotide sequence and the predicted amino acid sequence of sbGH was determined from a recombinant done carrying the longest cDNA insert. The sbGH cDNA encodes a pre-hormone of 204 amino acid residues. Comparison of the predicted amino acid sequence of sbGH with those of other vertebrates revealed different degrees of sequence identity: approximately 98% with European sea bass; 90% with bluefin tuna; bonito and red seabream; 71% with winter flounder; 64% with salmonids; 55% withwp; and 38% with human. Expression of the mature sbGH cDNA (without the signal peptide sequence) in E. coli cells under regulation of the lambda phage PL promoter produced a polypeptide of 20 kDa. Following renaturation, this recombinant hormone was shown to be biologically active in a radioreceptor competition binding assay and in the induction of hepatic insulin-like growth factor I (IGF-I) mRNA synthesis in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:05:49