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Titolo:
PREVENTION OF CYTOKINE-INDUCED CHANGES IN LEUKOCYTE ADHESION RECEPTORS BY NONSTEROIDAL ANTIINFLAMMATORY DRUGS FROM THE OXICAM FAMILY
Autore:
GARCIAVICUNA R; DIAZGONZALEZ F; GONZALEZALVARO I; DELPOZO MA; MOLLINEDO F; CABANAS C; GONZALEZAMARO R; SANCHEZMADRID F;
Indirizzi:
UNIV AUTONOMA MADRID,HOSP LA PRINCESA,SERV IMMUNOL,C DIEGO DE LEON 62MADRID SPAIN UNIV AUTONOMA MADRID,HOSP LA PRINCESA,SERV IMMUNOL MADRID SPAIN UNIV VALLADOLID,INST MOL BIOL & GENET VALLADOLID SPAIN UNIV COMPLUTENSE MADRID MADRID SPAIN UNIV SAN LUIS POTOSI SAN LUIS POTOSI MEXICO
Titolo Testata:
Arthritis and rheumatism
fascicolo: 1, volume: 40, anno: 1997,
pagine: 143 - 153
SICI:
0004-3591(1997)40:1<143:POCCIL>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPIRIN-LIKE DRUGS; TUMOR-NECROSIS-FACTOR; T-CELL ADHESION; HUMAN-NEUTROPHILS; MONOCLONAL-ANTIBODY; CHEMOTACTIC FACTORS; SURFACE EXPRESSION; L-SELECTIN; ACTIVATION; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
R. Garciavicuna et al., "PREVENTION OF CYTOKINE-INDUCED CHANGES IN LEUKOCYTE ADHESION RECEPTORS BY NONSTEROIDAL ANTIINFLAMMATORY DRUGS FROM THE OXICAM FAMILY", Arthritis and rheumatism, 40(1), 1997, pp. 143-153

Abstract

Objective. To explore the effect of the nonsteroidal antiinflammatorydrugs (NSAIDs) piroxicam and meloxicam on quantitative and qualitative changes in leukocyte adhesion receptors induced by cytokines and other activation stimuli. Methods. The expression of CD11b and L-selectinduring neutrophil activation with tumor necrosis factor alpha (TNF alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), FMLP,phorbol myristate acetate (PMA), and calcium ionophore A23187 was assessed by flow cytometry. Enzyme-linked immunosorbent assays were used to quantitate soluble L-selectin shed after neutrophil stimulation. Enzyme release was measured to determine neutrophil degranulation by proinflammatory stimuli. Changes in affinity state of beta 1 and beta 2 integrins after neutrophil and T lymphocyte stimulation were assessed, by flow cytometry, using the monoclonal antibodies (MAb) HUTS-21 (anti-beta 1) and CBRM1/5 (anti-CD11b), which recognize activation-dependent epitopes on these two integrins. Results. Pretreatment of neutrophils with either NSAID prevented the changes in L-selectin and CD11b expression induced by TNF alpha, GM-CSF, and FMLP, but not those induced by PMA or A23187. Furthermore, piroxicam significantly decreased the amount of L-selectin shed by cytokine-treated neutrophils, whereas it did not exert this effect on PMA- or A23187-treated neutrophils. Piroxicam also decreased the release of gelatinase and lysozyme induced by TNF alpha, but not by PMA. Interestingly, piroxicam prevented the conformational changes that beta 2 integrins underwent upon activation of neutrophils: the appearance of the activation epitope of CD11b, detectedby the CBRM1/5 MAb, was blocked by piroxicam in TNF alpha-treated neutrophils. Moreover, in chemokine-treated T lymphocytes, the expressionof activation epitopes on beta 1 integrins was also diminished by piroxicam. In contrast, this NSAID did not affect the beta 1 integrin conformational changes induced by PMA or Mn++. Conclusion. Our results indicate that members of the oxicam family are able to interfere with events of neutrophil function, such as their degranulation and cytokine-mediated activation changes in adhesion molecules, both in neutrophilsand in lymphocytes. Such effects may significantly contribute to the antiinflammatory activity of these drugs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:23:12