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Titolo:
CUMULUS CELL-FUNCTION DURING BOVINE OOCYTE MATURATION, FERTILIZATION,AND EMBRYO DEVELOPMENT IN-VITRO
Autore:
ZHANG L; JIANG S; WOZNIAK PJ; YANG XZ; GODKE RA;
Indirizzi:
CORNELL UNIV,DEPT ANIM SCI,205 MORRISON HALL ITHACA NY 14853 CORNELL UNIV,DEPT ANIM SCI ITHACA NY 14853 LOUISIANA STATE UNIV,DEPT ANIM SCI BATON ROUGE LA 70803 LOUISIANA STATE UNIV,DEPT EXPTL STAT BATON ROUGE LA 70803
Titolo Testata:
Molecular reproduction and development
fascicolo: 3, volume: 40, anno: 1995,
pagine: 338 - 344
SICI:
1040-452X(1995)40:3<338:CCDBOM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURED INVITRO; FOLLICLE CELLS; MEIOTIC MATURATION; ACROSOME REACTION; BLASTOCYST STAGE; CULTURED INVITRO; GAP-JUNCTIONS; SOMATIC-CELLS; MOUSE OOCYTES; RAT OOCYTES;
Keywords:
ZYGOTES; CUMULUS-OOCYTE COMPLEXES; ZONA PELLUCIDA; GAP JUNCTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
L. Zhang et al., "CUMULUS CELL-FUNCTION DURING BOVINE OOCYTE MATURATION, FERTILIZATION,AND EMBRYO DEVELOPMENT IN-VITRO", Molecular reproduction and development, 40(3), 1995, pp. 338-344

Abstract

Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at variousstages. In humans, the cumulus cells are often removed after 15-18 hrof sperm-oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0, 7, 20, and 48 hr. The cumulus-free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, andblastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures. Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4-26% vs. 93-96%), fertilization (0-9% vs. 91-92%), and in Vitro development at all stages evaluated. Cumuluscells removed immediately prior to in Vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58-60% and 71%, respectively, vs. 91-92% for controls), cleavage development (40-47% and 53-54% vs. 74-78% for controls), and morula plus blastocyst development(15% and 24% vs. 45%, P < 0.05). Cumulus cell co-culture started at Various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05). Cumulus cell removal at 20 hr after IVF resulted in similar development to controls (P > 0.05) at all stages tested in this study. The intact state of surrounding cumulus cells of oocytes or embryos appears to be beneficial before or shortly after insemination (at or before 7 hr of IVF) but not essential at 20 hr after IVF. (C) 1995 Wiley-Liss, Inc.

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Documento generato il 07/07/20 alle ore 11:15:31