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Titolo:
CATHEPSIN-B AND CATHEPSIN-L IN ISOLATED PROXIMAL TUBULAR SEGMENTS DURING ACUTE AND CHRONIC PROTEINURIA
Autore:
EISENBERGER U; FELS LM; OLBRICHT CJ; STOLTE H;
Indirizzi:
HANNOVER MED SCH,KONSTANTY GUTSCHOW STR 8 D-30625 HANNOVER GERMANY HANNOVER MED SCH,DEPT INTERNAL MED,DIV NEPHROL W-3000 HANNOVER GERMANY
Titolo Testata:
Renal physiology and biochemistry
fascicolo: 2, volume: 18, anno: 1995,
pagine: 89 - 96
SICI:
1011-6524(1995)18:2<89:CACIIP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRIAMYCIN NEPHROSIS; RAT-KIDNEY; MWF RATS; DEGRADATION; CATABOLISM; AUTOPHAGY; GLOMERULI; STRAIN; CELLS;
Keywords:
CATHEPSIN PHYSIOLOGY; PROTEINURIA, ACUTE; PROTEINURIA, CHRONIC; PROTEIN REABSORPTION; PROXIMAL TUBULE; ADRIAMYCIN NEPHROPATHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
U. Eisenberger et al., "CATHEPSIN-B AND CATHEPSIN-L IN ISOLATED PROXIMAL TUBULAR SEGMENTS DURING ACUTE AND CHRONIC PROTEINURIA", Renal physiology and biochemistry, 18(2), 1995, pp. 89-96

Abstract

Acute and chronic proteinuria were studied in rats, using lysosomal cathepsin B and L as marker enzymes for tubular protein degradation. The activity of cathepsin B and L has been determined in microdissected segments S1, S2 and S3 of the proximal tubule by an ultramicroassay. Z-Phenylalanyl-arginine-7-amido-4-methylcoumarin served as a substrate. In normoproteinuric Sprague-Dawley rats, induction of acute unselective glomerular proteinuria with Adriamycin (5 mg/kg body weight) revealed a moderate activity increase of cathepsin B and L in the S2 segment, reaching 12.6+/-5.6 versus 8.6+/-4.2 pmol.mm(-1).min(-1) in controls. In contrast, Munich Wistar Fromter (MWF) rats, that are characterized by a genetically determined, chronically elevated glomerular proteinexcretion, showed a very high activity of cathepsin selectively in S2of 25.0+/-12.1 pmol.mm(-1).min(-1). Acute proteinuria induced by Adriamycin in chronic proteinuric MWF rats could increase cathepsin activity in the S3 segment only, showing 12.0+/-8.3 versus 6.8+/-4.0 pmol.mm(-1).min(-1) in MWF control rats. In conclusion, chronically increasedprotein filtration changes the functional reserve capacity of the proximal tubule. While acutely induced glomerular proteinuria in normoproteinuric rats stimulates lysosomal proteolytic activity mainly in S2 segment, chronic proteinuric MWF rats may display already a maximally stimulated cathepsin activity in this segment probably due to long-termincreased tubular protein load. In case of acute elevation of chronicproteinuria, the consecutive S3 segment shows increased lysosomal function for protein conservation.

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Documento generato il 26/09/20 alle ore 11:59:40