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Titolo:
COMPARISON OF 2 HLA-DRB HIGH-RESOLUTION MICROTITER PLATE REVERSE HYBRIDIZATION TYPING METHODS - ADVANTAGE OF A CODON-86 VALINE OR GLYCINE PCR SEGREGATION
Autore:
PEPONNET C; SCHAEFFER V; LEPAGE V; CHATELAIN F; RODDE I; ALSAYED J; BOUCHER P; HERMANS P; DELINVAL NMC; CHARRON D;
Indirizzi:
GENSET,1 RUE ROBERT & SONIA DELAUNAY F-75011 PARIS FRANCE HOP ST LOUIS,IMMUNOL & HISTOCOMPATIBIL LAB PARIS FRANCE
Titolo Testata:
Tissue antigens
fascicolo: 2, volume: 45, anno: 1995,
pagine: 129 - 138
SICI:
0001-2815(1995)45:2<129:CO2HHM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; GROUP-SPECIFIC AMPLIFICATION; OLIGONUCLEOTIDE PROBES; AMPLIFIED DNA; LABELED OLIGONUCLEOTIDES; ANTIGENS; PRIMERS; ALLELES; IDENTIFICATION; POLYMORPHISM;
Keywords:
CLASS II; HLA; MICROTITER PLATE; OLIGOTYPING; PCR; REVERSE HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C. Peponnet et al., "COMPARISON OF 2 HLA-DRB HIGH-RESOLUTION MICROTITER PLATE REVERSE HYBRIDIZATION TYPING METHODS - ADVANTAGE OF A CODON-86 VALINE OR GLYCINE PCR SEGREGATION", Tissue antigens, 45(2), 1995, pp. 129-138

Abstract

Two rapid, nonisotopic, high-resolution HLA-DRB typing methods have been developed for DRB1, DRB3, DRB4 and DRB5 alleles. These methods arebased on a single procedure consisting of the reverse hybridization of biotinylated amplicons to oligonucleotide probes that are covalentlyattached to a microtiter plate. Detection is by an enzymatic reactionwith a fluorescent substrate. The 1 Generic Amplification (1GA) method amplifies ail HLA-DRB alleles in the same reaction mix. The 2 Allelic Subset Amplification (2SA) method uses two distinct amplification reactions that distributes all DRB alleles into two equal-size subsets, according to the codon 86 Gly or Val polymorphism; this adds an extra discrimination level to the typing. 108 samples were typed using the 1GA and the 2SA methods and no discrepancies were found. Typing indeterminations due to overlapping probe combinations were compared; it was found that the 2SA method, with the extra discrimination level at the PCR step, greatly improved resolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:19:01