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Titolo:
STRUCTURAL-STUDIES AND FUNCTIONAL-STUDIES OF RETROVIRAL RNA PSEUDOKNOTS INVOLVED IN RIBOSOMAL FRAMESHIFTING - NUCLEOTIDES AT THE JUNCTION OF THE 2 STEMS ARE IMPORTANT FOR EFFICIENT RIBOSOMAL FRAMESHIFTING
Autore:
CHEN XY; CHAMORRO M; LEE SI; SHEN LX; HINES JV; TINOCO I; VARMUS HE;
Indirizzi:
UNIV CALIF BERKELEY,DEPT CHEM BERKELEY CA 94720 UNIV CALIF BERKELEY,CHEM BIODYNAM LAB BERKELEY CA 94720 NCI BETHESDA MD 20892 UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL & BIOCHEM & BIOPHYSSAN FRANCISCO CA 94143 BROWN UNIV,MIRIAM HOSP PROVIDENCE RI 02906 KENYON COLL,DEPT CHEM GAMBIER OH 43022
Titolo Testata:
EMBO journal
fascicolo: 4, volume: 14, anno: 1995,
pagine: 842 - 852
SICI:
0261-4189(1995)14:4<842:SAFORR>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-III HOLOENZYME; ESCHERICHIA-COLI; MESSENGER-RNA; GENE-EXPRESSION; FUSION PROTEIN; GAMMA-SUBUNIT; VIRUS; TRANSLATION; SITE; MECHANISM;
Keywords:
FRAMESHIFTING; NICKEL COMPLEX; PSEUDOKNOT; RETROVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
X.Y. Chen et al., "STRUCTURAL-STUDIES AND FUNCTIONAL-STUDIES OF RETROVIRAL RNA PSEUDOKNOTS INVOLVED IN RIBOSOMAL FRAMESHIFTING - NUCLEOTIDES AT THE JUNCTION OF THE 2 STEMS ARE IMPORTANT FOR EFFICIENT RIBOSOMAL FRAMESHIFTING", EMBO journal, 14(4), 1995, pp. 842-852

Abstract

Ribosomal frameshifting, a translational mechanism used during retroviral replication, involves a directed change in reading frame at a specific site at a defined frequency, Such programmed frameshifting at the mouse mammary tumor virus (MMTV) gag-pro shift site requires two mRNA signals: a heptanucleotide shifty sequence and a pseudoknot structure positioned downstream, Using in vitro translation assays and enzymatic and chemical probes for RNA structure, we have defined features of the pseudoknot that promote efficient frameshifting. Heterologous RNA structures, e.g, a hairpin, a tRNA or a synthetic pseudoknot, substituted downstream of the shifty site fail to promote frameshifting, suggesting that specific features of the MMTV pseudoknot are important for function. Site-directed mutations of the MMTV pseudoknot indicate thatthe pseudoknot junction, including an unpaired adenine nucleotide between the two stems, provides a specific structural determinant for efficient frameshifting, Pseudoknots derived from other retroviruses (i.e. the feline immunodeficiency virus and the simian retrovirus type 1) also promote frameshifting at the MMTV gag-pro shift site, dependent on the same structure at the junction of the two stems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:25:47