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Titolo:
THE INTERFERON-INDUCED MX-PROTEIN OF CHICKENS LACKS ANTIVIRAL ACTIVITY
Autore:
BERNASCONI D; SCHULTZ U; STAEHELI P;
Indirizzi:
UNIV FREIBURG,INST MIKROBIOL & HYG,DEPT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,INST MIKROBIOL & HYG,DEPT VIROL D-79008 FREIBURG GERMANY
Titolo Testata:
Journal of interferon & cytokine research
fascicolo: 1, volume: 15, anno: 1995,
pagine: 47 - 53
SICI:
1079-9907(1995)15:1<47:TIMOCL>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS-VIRUS; INFLUENZA-VIRUS; GENE PROMOTER; RESISTANCE; EXPRESSION; GTPASE; INHIBITION; CONTAIN; MURINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
D. Bernasconi et al., "THE INTERFERON-INDUCED MX-PROTEIN OF CHICKENS LACKS ANTIVIRAL ACTIVITY", Journal of interferon & cytokine research, 15(1), 1995, pp. 47-53

Abstract

cDNA sequencing revealed that chick Mr protein consists of 705 amino acids, Its 84 N-terminal amino acids show no significant sequence homology to other Mr proteins, They are followed by 514 residues that include a tripartite GTP binding consensus moth, This region shows 50-70% sequence identity to mammalian and duck Mr proteins, Sequences near the C terminus, including a leucine zipper motif, are also conserved, whereas the intervening 19 amino acids lack sequence similarity, This unique sequence corresponds to a highly variable region in mammalian Mr proteins, suggesting that it serves as a spacer between functional domains, Chick and mouse cells transiently transfected with cDNA expression constructs synthesized chick Mr protein at a level that could easily be detected with specific antibodies, Chick Mr protein in such cellswas mainly cytoplasmic and had a granular appearance, Permanently transfected cell lines expressing high levels of chick Mr protein could not be established, suggesting low metabolic stability of chick Mr protein or incompatibility with cell proliferation, The antiviral activityof chick Mr protein was tested at the single-cell level using immunofluorescence techniques, Transfected cells expressing chick Mr protein showed no enhanced resistance to influenza A virus, vesicular stomatitis virus, Thogoto virus, or Sendai virus, Thus, chick Mr joins the list of Mr proteins without recognized antiviral activity, supporting theconcept that Mr proteins serve unrelated functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 02:26:27