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Titolo:
AN HPLC METHOD TO STUDY THE UPTAKE AND PARTITIONING OF THE ANTIOZONANT EDU IN BEAN-PLANTS
Autore:
REGNERJOOSTEN K; MANDERSCHEID R; BERGMANN E; BAHADIR M; WEIGEL HJ;
Indirizzi:
BUNDESFORSCHUNGSANSTALT LANDWIRTSCHAFT,INST PRODUKT & OKOTOXIKOL,BUNDESALLEE 50 D-38116 BRAUNSCHWEIG GERMANY
Titolo Testata:
Angewandte Botanik
fascicolo: 5-6, volume: 68, anno: 1994,
pagine: 151 - 155
SICI:
0066-1759(1994)68:5-6<151:AHMTST>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
K. Regnerjoosten et al., "AN HPLC METHOD TO STUDY THE UPTAKE AND PARTITIONING OF THE ANTIOZONANT EDU IN BEAN-PLANTS", Angewandte Botanik, 68(5-6), 1994, pp. 151-155

Abstract

The chemical compound EDU -[2-(2-oxo-1-imidazolidinyl)-ethyl]-N'-phenylurea) has long been used as an antiozonant to protect plants from ozone damage. However, as there is no assay available to determine its concentration in plant tissues its mode of application is still a matter of controversy. In the present study the uptake and distribution of EDU in bean plants (Phaseolus vulgaris L.) grown hydroponically in thegreenhouse (August-September and January-February) with 3 mg EDU l-1 nutrient solution was investigated. EDU was analysed by a newly developed HPLC technique using reversed phase separation and UV-detection at240 nm. Two methods were developed for the quantitative analysis of EDU in the nutrient solution and in different plant organs, respectively, and the identification of the EDU peak in the various samples was confirmed by the UV spectrum recorded with a photodiodearray detector. EDU could be detected in the range of 8-700 ng. There was no change ofthe EDU concentration in the nutrient solution without plants and with plant culture indicating the compound to be taken up with the nutrient solution. EDU was also detected in the stem exudate of decapitated plants. After two weeks of EDU treatment, only 4% of the EDU lost fromthe nutrient solution were found in the plant tissue. Of the EDU content of a total plant (63 mug) 17% were found in roots, 4% in stems and79% in leaves. The EDU concentration was highest in the intercellularwashing fluid of the leaves and values for the apoplastic fluid were calculated to be about 37 mg l-1 and 15 mg l-1 in plants grown in summer and winter, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/10/20 alle ore 10:36:26