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Titolo:
THE SMALL GTP-BINDING PROTEIN RAB7 IS ESSENTIAL FOR CELLULAR VACUOLATION INDUCED BY HELICOBACTER-PYLORI CYTOTOXIN
Autore:
PAPINI E; SATIN B; BUCCI C; DEBERNARD M; TELFORD JL; MANETTI R; RAPPUOLI R; ZERIAL M; MONTECUCCO C;
Indirizzi:
UNIV PADUA,CNR,CTR BIOMEMBRANE,VIA TRIESTE 75 I-35121 PADUA ITALY UNIV PADUA,DIPARTIMENTO SCI BIOMED I-35121 PADUA ITALY UNIV NAPLES FEDERICO II,DIPARIMENTO BIOL & PATOL MOL & CELLULARE L CALIFA I-80131 NAPLES ITALY CNR,CTR ENDOCRINOL & ONCOL SPERIMENTALE I-80131 NAPLES ITALY IRIS I-53100 SIENA ITALY EUROPEAN MOL BIOL LAB D-69012 HEIDELBERG GERMANY
Titolo Testata:
EMBO journal
fascicolo: 1, volume: 16, anno: 1997,
pagine: 15 - 24
SICI:
0261-4189(1997)16:1<15:TSGPRI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; VESICULAR TRANSPORT; LATE ENDOSOMES; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; MUTATIONAL ANALYSIS; ACTIVATING PROTEIN; HELA-CELLS; FUSION; TOXIN;
Keywords:
CYTOTOXIN; GASTRIC ULCER; HELICOBACTER PYLORI; LATE ENDOSOMES; RAB7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
E. Papini et al., "THE SMALL GTP-BINDING PROTEIN RAB7 IS ESSENTIAL FOR CELLULAR VACUOLATION INDUCED BY HELICOBACTER-PYLORI CYTOTOXIN", EMBO journal, 16(1), 1997, pp. 15-24

Abstract

The VacA cytotoxin, produced by toxigenic strains of Helicobacter pylori, induces the formation of large vacuoles highly enriched in the small GTPase rab7. To probe the role of rab7 in vacuolization, HeLa cells were transfected with a series of rab mutants and exposed to VacA. Dominant-negative mutants of rab7 effectively prevented vacuolization, whereas homologous rab5 and rab9 mutants were only partially inhibitory or ineffective, respectively. Expression of wild-type or GTPase-deficient rab mutants synergized with VacA in inducing vacuolization. In vitro fusion of late endosomes was enhanced by active rab7 and inhibited by inactive rab7, consistent with vacuole formation by merging of late endosomes in a process that requires functional rab7. Taken together, the effects of overexpressed rab proteins described here indicate that continuous membrane flow along the endocytic pathway is necessary for vacuole growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 09:20:03