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Titolo:
PRESENCE, ACTIVITIES, AND MOLECULAR-FORMS OF CATHEPSIN-G, ELASTASE, ALPHA(1)-ANTITRYPSIN, AND ALPHA(1)-ANTICHYMOTRYPSIN IN BRONCHIECTASIS
Autore:
SEPPER R; KONTTINEN YT; INGMAN T; SORSA T;
Indirizzi:
UNIV HELSINKI,DEPT ANAT,MOLEC BIOL LAB,POB 9 SF-00014 HELSINKI FINLAND TARTU STATE UNIV,LUNG CLIN TARTU 202400 ESTONIA UNIV HELSINKI,DEPT PERIODONTOL HELSINKI FINLAND UNIV HELSINKI,DEPT MED CHEM HELSINKI FINLAND
Titolo Testata:
Journal of clinical immunology
fascicolo: 1, volume: 15, anno: 1995,
pagine: 27 - 34
SICI:
0271-9142(1995)15:1<27:PAAMOC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN NEUTROPHIL ELASTASE; OXIDIZED ALPHA-1-PROTEINASE INHIBITOR; LEUKOCYTE ELASTASE; HUMAN ALPHA-1-ANTICHYMOTRYPSIN; ELASTOLYTIC ACTIVITY; LUNG-DISEASE; PROTEINASES; DEGRADATION; COLLAGENASE; INACTIVATION;
Keywords:
BRONCHIECTASIS; BRONCHOALVEOLAR LAVAGE FLUID; POLYMORPHONUCLEAR LEUKOCYTE ELASTASE; CATHEPSIN G; ALPHA(1)-ANTITRYPSIN; ALPHA(1)-ANTICHYMOTRYPSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
R. Sepper et al., "PRESENCE, ACTIVITIES, AND MOLECULAR-FORMS OF CATHEPSIN-G, ELASTASE, ALPHA(1)-ANTITRYPSIN, AND ALPHA(1)-ANTICHYMOTRYPSIN IN BRONCHIECTASIS", Journal of clinical immunology, 15(1), 1995, pp. 27-34

Abstract

The presence, activities, and molecular forms of the serine proteinases, elastase, and cathepsin G, and their endogenous inhibitors, alpha(1)-antitrypsin and alpha(1)-antichymotrypsin, were investigated in bronchoalveolar lavage (BAL) fluid of bronchiectasis patients divided into mild, moderate, and severe disease subgroups and compared to BAL fluid from healthy controls. Immunochemical characterization and quantitation were performed by Western immunoblot. The activities of elastase and cathepsin G were recorded spectrophotometrically using synthetic substrates. The results showed a significant difference in elastase andcathepsin G activities in BAL fluid of the three subgroups, revealingthe following data-mild subgroup, 0.21 +/- 0.09 mU/g and 57.35 +/- 20.9 U/g; moderate subgroup, 1.87 +/- 1.12 mU/g and 89.24 +/- 31.4 U/g; and severe subgroup, 2.63 +/- 1.63 mU/g and 139.18 +/- 58.3 U/g, respectively-compared to those of the healthy control group, 0.09 +/- 0.03 mU/g and 50.96 +/- 16.5 U/g. Evidently, the protective shield of plasma-derived antiproteinases was sufficient in healthy subjects and, also, in mild cases of bronchiectasis, but not in cases of severe and moderate forms of bronchiectasis, in which free and catalytically active elastase and cathepsin G were detected. The serine proteinases inhibitors (serpins), alpha(1)-antitrypsin and alpha(1)-antichymotrypsin, haveevidently been oxidized and/or proteolytically cleaved in the cases of moderate and severe bronchiectasis. The results indicate that insufficient endogenous downregulation of catalytically active elastase and cathepsin G in BALE leads to tissue injury, resulting in alterative and deformative processes in the bronchiectasis lung.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 04:45:42