Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EXPRESSION OF ICAM-1 AND VCAM-1 AND MONOCYTE ADHERENCE IN ARTERIES EXPOSED TO ALTERED SHEAR-STRESS
Autore:
WALPOLA PL; GOTLIEB AI; CYBULSKY MI; LANGILLE BL;
Indirizzi:
TORONTO HOSP,RES INST,MAX BELL RES CTR,200 ELIZABETH ST TORONTO ON M5G 2C4 CANADA TORONTO HOSP,RES INST,MAX BELL RES CTR TORONTO ON M5G 2C4 CANADA UNIV TORONTO,BANTING & BEST DIABET CTR TORONTO ON M5G 1L5 CANADA UNIV TORONTO,CARDIOVASC RES CTR,DEPT PATHOL TORONTO ON CANADA BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV VASC RES BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 00000
Titolo Testata:
Arteriosclerosis, thrombosis, and vascular biology
fascicolo: 1, volume: 15, anno: 1995,
pagine: 2 - 10
SICI:
1079-5642(1995)15:1<2:EOIAVA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE ADHESION MOLECULE; HUMAN ENDOTHELIAL-CELLS; MESSENGER-RNA LEVELS; INDUCED CALCIUM TRANSIENTS; VASCULAR ENDOTHELIUM; NEUTROPHIL ADHESION; AORTIC ENDOTHELIUM; RABBIT AORTA; FLOW; VENULES;
Keywords:
ADHESION; ELAM; ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
P.L. Walpola et al., "EXPRESSION OF ICAM-1 AND VCAM-1 AND MONOCYTE ADHERENCE IN ARTERIES EXPOSED TO ALTERED SHEAR-STRESS", Arteriosclerosis, thrombosis, and vascular biology, 15(1), 1995, pp. 2-10

Abstract

Local shear stresses generated by blood flow exert direct mechanical effects on adhesion of circulating leukocytes to vascular endothelium,but their effects on expression of endothelial-leukocyte adhesion molecules have not been determined. Shear stress in rabbit carotid arteries was increased by 170% or decreased by 73% in 5 days by surgical manipulations. En face immunofluorescence staining with the monoclonal antibody Rb1/9 revealed that vascular cell adhesion molecule-1 (VCAM-1) expression was greatly increased under low shear stress, but the distribution of staining was patchy. Thus, 71.4+/-7.8% of fields were VCAM-1 positive versus 2.4+/-0.47% of fields in control arteries. Frequently, large regions showed consistent but heterogeneous staining. Occasionally, small islands of cells were labeled intensely. Monocytes, detected by use of the monocyte-specific antibody HAM 56, adhered to endothelium under low shear stress; 64.5+/-8.2% of the monocytes colocalizedwith detectable VCAM-1, although many (83.2+/-2.8%) VCAM-1-positive regions were devoid of monocytes. VCAM-1 expression also increased significantly but to a lesser extent when shear stress was approximately doubled. Thus, 8.7+/-1.5% of fields were VCAM-1 positive under high shear versus 2.5+/-0.87% under normal shear stress. No monocytes were detected at high shear stress. At normal shear stresses, intercellular adhesion molecule-1 (ICAM-1), detected by use of the monoclonal antibodyRb2/3, was extensively distributed; thus, 53.5+/-5.5% of fields contained ICAM-1-positive cells. The junctional regions of the cells were heavily stained. Experimental increases in shear stress significantly upregulated ICAM-1 expression (88.3+/-2.0% of fields were ICAM-1 positive; P<.05). Staining was again concentrated in the cell junctional regions. Reduced shear stress suppressed ICAM-1 expression (16.6+/-10.3% of fields were positive), and ICAM-1 was more commonly, but not exclusively, distributed diffusely. Junctional ICAM-1 may participate in endothelial cell-cell adhesion; alternatively, a pool of ICAM-1 concentrated between cell junctions may be inaccessible to circulating leukocytes until endothelial cell activation presents the molecule to the vessel lumen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:59:44