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Titolo:
READTHROUGH PROTEIN ASSOCIATED WITH VIRIONS OF BARLEY YELLOW DWARF LUTEOVIRUS AND ITS POTENTIAL ROLE IN REGULATING THE EFFICIENCY OF APHID TRANSMISSION
Autore:
WANG JY; CHAY C; GILDOW FE; GRAY SM;
Indirizzi:
CORNELL UNIV,USDA ARS ITHACA NY 14853 CORNELL UNIV,USDA ARS ITHACA NY 14853 CORNELL UNIV,DEPT PLANT PATHOL ITHACA NY 14853 PENN STATE UNIV,DEPT PLANT PATHOL UNIVERSITY PK PA 16802
Titolo Testata:
Virology
fascicolo: 2, volume: 206, anno: 1995,
pagine: 954 - 962
SICI:
0042-6822(1995)206:2<954:RPAWVO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE ANALYSIS; POTATO LEAFROLL VIRUS; MONOCLONAL-ANTIBODIES; GENES; RNA; IDENTIFICATION; ORGANIZATION; EXPRESSION; ISOLATE; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.Y. Wang et al., "READTHROUGH PROTEIN ASSOCIATED WITH VIRIONS OF BARLEY YELLOW DWARF LUTEOVIRUS AND ITS POTENTIAL ROLE IN REGULATING THE EFFICIENCY OF APHID TRANSMISSION", Virology, 206(2), 1995, pp. 954-962

Abstract

Purified particles of barley yellow dwarf luteovirus (BYDV) contain amajor 22-kDa protein and a minor protein of approximately 58 kDa. The22-kDa capsid protein is encoded by open reading frame (ORF) 3. ORF 5is immediately downstream and in frame with ORF 3 and a 72-kDa protein can be translated via a readthrough suppression of the ORF 3 termination codon. Antibodies were produced against two Escherichia coli expressed polypeptides that represent the amino- and carboxyl-terminal halves of a putative 50-kDa protein encoded by ORF 5. Immunological analyses indicated that the 58-kDa protein associated with purified virionscontained sequences encoded by ORF 3 and ORF 5. The carboxyl terminalportion of the full-length (72 kDa) readthrough protein was absent from the 58-kDa protein. The full-length readthrough protein was detected in infected oat protoplasts and plant tissue, but was not associatedwith virus particles purified from plants. The carboxyl-terminal portion of the 72-kDa readthrough protein was not required for aphid transmission; however, virus was transmitted more efficiently from protoplast extracts containing virions and soluble 72-kDa readthrough protein than from mock-inoculated protoplast extracts to which plant purified virus was added. The full-length readthrough protein, although not required for transmission, may increase the transmission efficiency of BYDV by aphids. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:29:45