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Titolo:
SIMPLE STRATEGY TO INDUCE ANTIBODIES OF DISTINCT SPECIFICITY - APPLICATION TO THE MAPPING OF GP120 AND INHIBITION OF HIV-1 INFECTIVITY
Autore:
PRIETO I; HERVASSTUBBS S; GARCIAGRANERO M; BERASAIN C; RIEZUBOJ JI; LASARTE JJ; SAROBE P; PRIETO J; BORRASCUESTA F;
Indirizzi:
UNIV NAVARRA,FAC MED,DEPT MED INTERNA,APARTADO 273 E-31008 PAMPLONA SPAIN UNIV NAVARRA,FAC MED,DEPT MED INTERNA E-31008 PAMPLONA SPAIN
Titolo Testata:
European Journal of Immunology
fascicolo: 4, volume: 25, anno: 1995,
pagine: 877 - 883
SICI:
0014-2980(1995)25:4<877:SSTIAO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; AMINO-ACID-SEQUENCE; B-CELL EPITOPE; NEUTRALIZING ANTIBODIES; SYNTHETIC PEPTIDES; ENVELOPE GLYCOPROTEINS; COVALENT LINKAGE; TYPE-1; DETERMINANT; IMMUNOGENICITY;
Keywords:
T HELPER CELL DETERMINANT; B CELL DETERMINANT; HIV-1; GP120; NEUTRALIZING ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
I. Prieto et al., "SIMPLE STRATEGY TO INDUCE ANTIBODIES OF DISTINCT SPECIFICITY - APPLICATION TO THE MAPPING OF GP120 AND INHIBITION OF HIV-1 INFECTIVITY", European Journal of Immunology, 25(4), 1995, pp. 877-883

Abstract

In this study 96 15-mer peptides encompassing the entire sequence of HIV-1 gp120 were synthesized and used to immunize BALB/c mice (i) alone or (ii) in conjunction with the T helper cell determinant FISEAIIHVLHSR (FIS) from sperm whale myoglobin, which is well recognized by major histocompatibility complex (MHC) class II molecules of BALB/c. Of these peptides 39 were immunogenic per se and 57 were not. Out of the 57non-immunogenic peptides 53 could be rendered immunogenic with the second immunization protocol. With the exception of 4 cases, the anti-peptide antibody titers induced in (ii) were equal (14 cases) or higher (78 cases) than those induced in (i). From the 96 anti-peptide antibodies tested, 12 were able to recognize recombinant gp120 with good antibody titers, a result in agreement with previously identified B cell epitopes from gp120 by anti-peptide antibodies induced with longer peptides conjugated to a carrier protein. Moreover, 4 of the 12 anti-peptide antisera that recognized gp120 were able to neutralize HIV-1 infectivity in vitro, showing that the strategy of co-immunization with FIS may afford functional antibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:33:42